Label-Free Imaging of Lipid Droplets in Live Cells: A Technical Note on Photodamage Thresholds

Abstract

There is real interest in label-free imaging of lipid droplets in live cells right now, and real uncertainty about exactly where it
delivers the most value. We pull together the current evidence for label-free imaging of lipid droplets in live cells, covering the
methodological, practical, regulatory and cost angles, with an eye on where practice has genuinely settled and where researchers
and reviewers are still largely working it out for themselves. Selective, context-specific adoption looks better supported by the
evidence gathered here than either broad rollout or blanket avoidance