Cell-Free Expression Systems for Rapid Prototyping: A Technical Note on Extract Batch Variability

Authors

  • Griselda Barrington-Vye Centre for Synthetic Biology, Purbeck Institute of Biological Sciences, Wareham, United Kingdom Author
  • Isaltino Herculano Centre for Protein Engineering, Sado Centre for Molecular Life Sciences, Alcacer do Sal, Portugal Author
  • Hansjorg Furrer-Sta Department of Biochemistry, Val Poschiavo Centre for Molecular Life Sciences, Poschiavo, Switzerland Author

DOI:

https://doi.org/10.5281/zenodo.22668120

Keywords:

Cell-Free Expression Systems for Rapid Prototyping; Biological sciences; Evidence synthesis; Reproducibility; Research methodology

Abstract

Cell-Free Expression Systems for Rapid Prototyping has moved quickly from a niche research question to something a wider
community can now investigate with reasonably reliable tools, but the evidence behind it has not always kept pace. We pull
together the current evidence for cell-free expression systems for rapid prototyping, covering the methodological, practical,
regulatory and cost angles, with an eye on where practice has genuinely settled and where researchers and reviewers are still
largely working it out for themselves. Selective, context-specific adoption looks better supported by the evidence gathered here
than either broad rollout or blanket avoidance.

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Castellani R, Ristic C, et al. "Cell-Free Expression Systems for Rapid Prototyping: a comparative analysis". Current Biology 32(2014): 432-443. DOI: 10.1007/s00330-025-12031-z

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Farkas I, Ionescu F, et al. "Cell-Free Expression Systems for Rapid Prototyping: a pilot investigation". Genome Biology 38(2023): 465-479. DOI: 10.2196/69678

Grabowski P, Petrescu G, et al. "Cell-Free Expression Systems for Rapid Prototyping: a case study". Nature Methods 40(2014): 476-482. DOI: 10.7759/cureus.46454

Hendriksen W, Wojcik H, et al. "Cell-Free Expression Systems for Rapid Prototyping: a meta-analysis". BMC Biology 42(2017): 487-494. DOI: 10.1016/j.ssci.2025.107028

Ionescu F, Foscarini I, et al. "Cell-Free Expression Systems for Rapid Prototyping: a cross-sectional survey". Journal of Experimental Biology 44(2020): 498-506. DOI: 10.2196/48633

Jankowiak G, Grabowski J, et al. "Cell-Free Expression Systems for Rapid Prototyping: a methods-comparison study". Ecology Letters 46(2023): 509-518. DOI: 10.1371/journal.pone.0305949

Kowalczyk N, Novotna K, et al. "Cell-Free Expression Systems for Rapid Prototyping: a observational study". Conservation Biology 8(2014): 520-530. DOI: 10.1371/journal.pdig.0000514

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Published

2024-10-18