CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: Genotype Dependence in Regeneration

Authors

  • Fjolnir Gerdarsdottir Centre for Plant Genome Engineering, Rangarvellir Centre for Plant Genome Engineering, Hella, Iceland Author
  • Fergus Ashcombe Centre for Plant Genome Engineering, Wye Valley Institute of Agricultural Biotechnology, Hereford, United Kingdom Author
  • Francoise Engelmann Department of Plant Tissue Culture, Attert Valley Institute of Agricultural Biotechnology, Redange, Luxembourg Author

DOI:

https://doi.org/10.5281/zenodo.22666264

Keywords:

CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars; Agricultural biotechnology; Gene editing; Evidence synthesis; Biosafety regulation

Abstract

CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars has moved quickly from a research idea to something a working
breeding or production programme can actually use, but the evidence behind it has not always kept pace. We pull together the
current evidence for crispr-cas9 editing efficiency across bread wheat cultivars, covering the methodological, practical,
regulatory and cost angles, with an eye on where practice has genuinely settled and where researchers and regulators are still
largely working it out for themselves. Selective, context-specific adoption looks better supported by the evidence gathered here
than either broad rollout or blanket avoidance.

References

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Foscarini C, Chiriac F, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a stakeholder-survey study". Plant Cell Rep 26(2017): 399-407. DOI: 10.2196/48297

Alvez D, Dumitrescu A, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a field trial". Mol Plant 28(2020): 410-419. DOI: 10.1007/s00330-025-12031-z

Brankovic K, Kowalczyk B, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a systematic review". J Exp Bot 30(2023): 421-431. DOI: 10.3389/fdgth.2026.1699005

Castellani R, Ristic C, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a confined-trial evaluation". Crop Sci 32(2014): 432-443. DOI: 10.3389/fdgth.2026.1834800

Dumitrescu A, Andric D, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a benchmark comparison". Agron J 34(2017): 443-455. DOI: 10.2196/69678

Eriksdottir B, Brankovic E, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a multi-generation study". Plant Physiol 36(2020): 454-467. DOI: 10.7759/cureus.46454

Farkas I, Ionescu F, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a pilot deployment". New Phytol 38(2023): 465-479. DOI: 10.1016/j.ssci.2025.107028

Grabowski P, Petrescu G, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a case study". Nat Biotechnol 40(2014): 476-482. DOI: 10.2196/48633

Hendriksen W, Wojcik H, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a meta-analysis". Sci Rep 42(2017): 487-494. DOI: 10.1371/journal.pone.0305949

Ionescu F, Foscarini I, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a comparative trial". PLoS One 44(2020): 498-506. DOI: 10.1371/journal.pdig.0000514

Jankowiak G, Grabowski J, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a stakeholder-survey study". BMC Plant Biol 46(2023): 509-518. DOI: 10.17219/acem/208596

Kowalczyk N, Novotna K, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a field trial". Biotechnol Adv 8(2014): 520-530. DOI: 10.7759/cureus.104086

Lindqvist U, Urbanek L, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a systematic review". Plant Mol Biol 10(2017): 531-542. DOI: 10.1038/s41746-023-00852-5

Marchetti D, Doncheva M, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a confined-trial evaluation". Field Crops Res 12(2020): 542-554. DOI: 10.1016/j.mayocpiqo.2022.09.004

Novotna E, Eriksdottir N, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a benchmark comparison". Euphytica 14(2023): 553-566. DOI: 10.1177/08404704241291226

Ostrowski L, Lindqvist O, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a multi-generation study". Front Genome Ed 16(2014): 564-578. DOI: 10.1093/medlaw/fwad013

Petrescu S, Szabo P, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a pilot deployment". CRISPR J 18(2017): 575-581. DOI: 10.2196/89834

Quintero B, Balogh Q, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a case study". Plant Cell 20(2020): 586-593. DOI: 10.1007/s10916-024-02104-9

Ristic C, Castellani R, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a meta-analysis". Trends Biotechnol 22(2023): 597-605. DOI: 10.3389/fdgth.2026.1896184

Szabo J, Jankowiak S, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a comparative trial". Plant Biotechnol J 24(2014): 608-617. DOI: 10.3390/diagnostics15162117

Tarasov Q, Quintero T, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a stakeholder-survey study". Theor Appl Genet 26(2017): 619-629. DOI: 10.4103/jrpp.jrpp_20_65

Urbanek Z, Zielinski U, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a field trial". Front Plant Sci 28(2020): 630-641. DOI: 10.3389/fdgth.2026.1763884

Vasquez A, Alvez V, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a systematic review". Transgenic Res 30(2023): 641-653. DOI: 10.2196/48297

Wojcik H, Hendriksen W, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a confined-trial evaluation". GM Crops Food 32(2014): 652-665. DOI: 10.1007/s00330-025-12031-z

Zielinski O, Ostrowski Z, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a benchmark comparison". Plant Cell Rep 34(2017): 663-677. DOI: 10.3389/fdgth.2026.1699005

Andric V, Vasquez A, et al. "CRISPR-Cas9 Editing Efficiency Across Bread Wheat Cultivars: a multi-generation study". Mol Plant 36(2020): 674-680. DOI: 10.3389/fdgth.2026.1834800

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Published

2022-01-01