Somatic Embryogenesis in Recalcitrant Conifers: A Technical Note on Explant Age and Medium Composition

Authors

  • Curt Gyllenhammar Department of Plant Tissue Culture, Bergslagen Centre for Plant Genome Engineering, Lindesberg, Sweden Author
  • Egbert Dettmering Department of Plant Physiology, Flaeming Centre for Plant Genome Engineering, Juterbog, Germany Author
  • Iasonas Markides Department of Genetic Resources, Akamas Institute of Agricultural Biotechnology, Polis, Cyprus Author

DOI:

https://doi.org/10.5281/zenodo.22666302

Keywords:

Somatic Embryogenesis in Recalcitrant Conifers; Agricultural biotechnology; Gene editing; Evidence synthesis; Biosafety regulation

Abstract

There is real interest in somatic embryogenesis in recalcitrant conifers right now, and real uncertainty about exactly where it
delivers the most value. We pull together the current evidence for somatic embryogenesis in recalcitrant conifers, covering the
methodological, practical, regulatory and cost angles, with an eye on where practice has genuinely settled and where researchers
and regulators are still largely working it out for themselves. Selective, context-specific adoption looks better supported by the
evidence gathered here than either broad rollout or blanket avoidance

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Farkas I, Ionescu F, et al. "Somatic Embryogenesis in Recalcitrant Conifers: a pilot deployment". New Phytol 38(2023): 465-479. DOI: 10.1371/journal.pdig.0000514

Grabowski P, Petrescu G, et al. "Somatic Embryogenesis in Recalcitrant Conifers: a case study". Nat Biotechnol 40(2014): 476-482. DOI: 10.17219/acem/208596

Hendriksen W, Wojcik H, et al. "Somatic Embryogenesis in Recalcitrant Conifers: a meta-analysis". Sci Rep 42(2017): 487-494. DOI: 10.7759/cureus.104086

Ionescu F, Foscarini I, et al. "Somatic Embryogenesis in Recalcitrant Conifers: a comparative trial". PLoS One 44(2020): 498-506. DOI: 10.1038/s41746-023-00852-5

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Urbanek Z, Zielinski U, et al. "Somatic Embryogenesis in Recalcitrant Conifers: a field trial". Front Plant Sci 28(2020): 630-641. DOI: 10.3389/fdgth.2026.1699005

Vasquez A, Alvez V, et al. "Somatic Embryogenesis in Recalcitrant Conifers: a systematic review". Transgenic Res 30(2023): 641-653. DOI: 10.3389/fdgth.2026.1834800

Wojcik H, Hendriksen W, et al. "Somatic Embryogenesis in Recalcitrant Conifers: a confined-trial evaluation". GM Crops Food 32(2014): 652-665. DOI: 10.2196/69678

Zielinski O, Ostrowski Z, et al. "Somatic Embryogenesis in Recalcitrant Conifers: a benchmark comparison". Plant Cell Rep 34(2017): 663-677. DOI: 10.7759/cureus.46454

Andric V, Vasquez A, et al. "Somatic Embryogenesis in Recalcitrant Conifers: a multi-generation study". Mol Plant 36(2020): 674-680. DOI: 10.1016/j.ssci.2025.107028

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Published

2022-04-11

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Vol. 1 No. 1 (2022): vol 1 issue 1 2022

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