Detection of Edited Alleles in Grain Consignments: A Benchmark of Digital PCR Against Sequencing

Authors

  • Ivo Galic Department of Analytical Science, Dalmatinska Zagora Centre for Plant Genome Engineering, Knin, Croatia Author
  • Gustav-Jan Bezdicek Centre for Plant Genome Engineering, Trebonsko Institute of Agricultural Biotechnology, Trebon, Czech Republic Author
  • Benita Celmins Centre for Regulatory Science, Talsi Centre for Plant Genome Engineering, Talsi, Latvia Author

DOI:

https://doi.org/10.5281/zenodo.22666314

Keywords:

Detection of Edited Alleles in Grain Consignments; Agricultural biotechnology; Gene editing; Evidence synthesis; Biosafety regulation

Abstract

Detection of Edited Alleles in Grain Consignments has moved quickly from a research idea to something a working breeding or
production programme can actually use, but the evidence behind it has not always kept pace. We pull together the current
evidence for detection of edited alleles in grain consignments, covering the methodological, practical, regulatory and cost angles,
with an eye on where practice has genuinely settled and where researchers and regulators are still largely working it out for
themselves. Selective, context-specific adoption looks better supported by the evidence gathered here than either broad rollout or
blanket avoidance.

References

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Foscarini C, Chiriac F, et al. "Detection of Edited Alleles in Grain Consignments: a stakeholder-survey study". Plant Cell Rep 26(2017): 399-407. DOI: 10.2196/69678

Alvez D, Dumitrescu A, et al. "Detection of Edited Alleles in Grain Consignments: a field trial". Mol Plant 28(2020): 410-419. DOI: 10.7759/cureus.46454

Brankovic K, Kowalczyk B, et al. "Detection of Edited Alleles in Grain Consignments: a systematic review". J Exp Bot 30(2023): 421-431. DOI: 10.1016/j.ssci.2025.107028

Castellani R, Ristic C, et al. "Detection of Edited Alleles in Grain Consignments: a confined-trial evaluation". Crop Sci 32(2014): 432-443. DOI: 10.2196/48633

Dumitrescu A, Andric D, et al. "Detection of Edited Alleles in Grain Consignments: a benchmark comparison". Agron J 34(2017): 443-455. DOI: 10.1371/journal.pone.0305949

Eriksdottir B, Brankovic E, et al. "Detection of Edited Alleles in Grain Consignments: a multi-generation study". Plant Physiol 36(2020): 454-467. DOI: 10.1371/journal.pdig.0000514

Farkas I, Ionescu F, et al. "Detection of Edited Alleles in Grain Consignments: a pilot deployment". New Phytol 38(2023): 465-479. DOI: 10.17219/acem/208596

Grabowski P, Petrescu G, et al. "Detection of Edited Alleles in Grain Consignments: a case study". Nat Biotechnol 40(2014): 476-482. DOI: 10.7759/cureus.104086

Hendriksen W, Wojcik H, et al. "Detection of Edited Alleles in Grain Consignments: a meta-analysis". Sci Rep 42(2017): 487-494. DOI: 10.1038/s41746-023-00852-5

Ionescu F, Foscarini I, et al. "Detection of Edited Alleles in Grain Consignments: a comparative trial". PLoS One 44(2020): 498-506. DOI: 10.1016/j.mayocpiqo.2022.09.004

Jankowiak G, Grabowski J, et al. "Detection of Edited Alleles in Grain Consignments: a stakeholder-survey study". BMC Plant Biol 46(2023): 509-518. DOI: 10.1177/08404704241291226

Kowalczyk N, Novotna K, et al. "Detection of Edited Alleles in Grain Consignments: a field trial". Biotechnol Adv 8(2014): 520-530. DOI: 10.1093/medlaw/fwad013

Lindqvist U, Urbanek L, et al. "Detection of Edited Alleles in Grain Consignments: a systematic review". Plant Mol Biol 10(2017): 531-542. DOI: 10.2196/89834

Marchetti D, Doncheva M, et al. "Detection of Edited Alleles in Grain Consignments: a confined-trial evaluation". Field Crops Res 12(2020): 542-554. DOI: 10.1007/s10916-024-02104-9

Novotna E, Eriksdottir N, et al. "Detection of Edited Alleles in Grain Consignments: a benchmark comparison". Euphytica 14(2023): 553-566. DOI: 10.3389/fdgth.2026.1896184

Ostrowski L, Lindqvist O, et al. "Detection of Edited Alleles in Grain Consignments: a multi-generation study". Front Genome Ed 16(2014): 564-578. DOI: 10.3390/diagnostics15162117

Petrescu S, Szabo P, et al. "Detection of Edited Alleles in Grain Consignments: a pilot deployment". CRISPR J 18(2017): 575-581. DOI: 10.4103/jrpp.jrpp_20_65

Quintero B, Balogh Q, et al. "Detection of Edited Alleles in Grain Consignments: a case study". Plant Cell 20(2020): 586-593. DOI: 10.3389/fdgth.2026.1763884

Ristic C, Castellani R, et al. "Detection of Edited Alleles in Grain Consignments: a meta-analysis". Trends Biotechnol 22(2023): 597-605. DOI: 10.2196/48297

Szabo J, Jankowiak S, et al. "Detection of Edited Alleles in Grain Consignments: a comparative trial". Plant Biotechnol J 24(2014): 608-617. DOI: 10.1007/s00330-025-12031-z

Tarasov Q, Quintero T, et al. "Detection of Edited Alleles in Grain Consignments: a stakeholder-survey study". Theor Appl Genet 26(2017): 619-629. DOI: 10.3389/fdgth.2026.1699005

Urbanek Z, Zielinski U, et al. "Detection of Edited Alleles in Grain Consignments: a field trial". Front Plant Sci 28(2020): 630-641. DOI: 10.3389/fdgth.2026.1834800

Vasquez A, Alvez V, et al. "Detection of Edited Alleles in Grain Consignments: a systematic review". Transgenic Res 30(2023): 641-653. DOI: 10.2196/69678

Wojcik H, Hendriksen W, et al. "Detection of Edited Alleles in Grain Consignments: a confined-trial evaluation". GM Crops Food 32(2014): 652-665. DOI: 10.7759/cureus.46454

Zielinski O, Ostrowski Z, et al. "Detection of Edited Alleles in Grain Consignments: a benchmark comparison". Plant Cell Rep 34(2017): 663-677. DOI: 10.1016/j.ssci.2025.107028

Andric V, Vasquez A, et al. "Detection of Edited Alleles in Grain Consignments: a multi-generation study". Mol Plant 36(2020): 674-680. DOI: 10.2196/48633

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Published

2022-05-30