Virus-Induced Gene Silencing for Functional Screening in Legumes: A Technical Note on Vector Choice

Authors

  • Ansgar Bollenbeck Department of Plant Virology, Kaiserstuhl Institute of Plant Science, Breisach, Germany Author
  • Guntram Dorfinger Department of Plant Molecular Biology, Sudburgenland Institute of Agricultural Biotechnology, Oberwart, Austria Author
  • Freddy Kalvet Centre for Crop Genomics, Alutaguse Institute of Agricultural Biotechnology, Johvi, Estonia Author

DOI:

https://doi.org/10.5281/zenodo.22666441

Keywords:

Virus-Induced Gene Silencing for Functional Screening in Legumes; Agricultural biotechnology; Gene editing; Evidence synthesis; Biosafety regulation

Abstract

There is real interest in virus-induced gene silencing for functional screening in legumes right now, and real uncertainty about
exactly where it delivers the most value. We pull together the current evidence for virus-induced gene silencing for functional
screening in legumes, covering the methodological, practical, regulatory and cost angles, with an eye on where practice has
genuinely settled and where researchers and regulators are still largely working it out for themselves. Selective, context-specific
adoption looks better supported by the evidence gathered here than either broad rollout or blanket avoidance.

References

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Brankovic K, Kowalczyk B, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a systematic review". Theor Appl Genet 10(2017): 91-98. DOI: 10.3389/fdgth.2026.1834800

Castellani R, Ristic C, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a confined-trial evaluation". Front Plant Sci 12(2020): 102-110. DOI: 10.2196/69678

Dumitrescu A, Andric D, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a benchmark comparison". Transgenic Res 14(2023): 113-122. DOI: 10.7759/cureus.46454

Eriksdottir B, Brankovic E, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a multi-generation study". GM Crops Food 16(2014): 124-134. DOI: 10.1016/j.ssci.2025.107028

Farkas I, Ionescu F, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a pilot deployment". Plant Cell Rep 18(2017): 135-146. DOI: 10.2196/48633

Grabowski P, Petrescu G, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a case study". Mol Plant 20(2020): 146-158. DOI: 10.1371/journal.pone.0305949

Hendriksen W, Wojcik H, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a meta-analysis". J Exp Bot 22(2023): 157-170. DOI: 10.1371/journal.pdig.0000514

Ionescu F, Foscarini I, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a comparative trial". Crop Sci 24(2014): 168-182. DOI: 10.17219/acem/208596

Jankowiak G, Grabowski J, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a stakeholder-survey study". Agron J 26(2017): 179-185. DOI: 10.7759/cureus.104086

Kowalczyk N, Novotna K, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a field trial". Plant Physiol 28(2020): 190-197. DOI: 10.1038/s41746-023-00852-5

Lindqvist U, Urbanek L, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a systematic review". New Phytol 30(2023): 201-209. DOI: 10.1016/j.mayocpiqo.2022.09.004

Marchetti D, Doncheva M, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a confined-trial evaluation". Nat Biotechnol 32(2014): 212-221. DOI: 10.1177/08404704241291226

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Petrescu S, Szabo P, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a pilot deployment". BMC Plant Biol 38(2023): 245-257. DOI: 10.1007/s10916-024-02104-9

Quintero B, Balogh Q, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a case study". Biotechnol Adv 40(2014): 256-269. DOI: 10.3389/fdgth.2026.1896184

Ristic C, Castellani R, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a meta-analysis". Plant Mol Biol 42(2017): 267-281. DOI: 10.3390/diagnostics15162117

Szabo J, Jankowiak S, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a comparative trial". Field Crops Res 44(2020): 278-284. DOI: 10.4103/jrpp.jrpp_20_65

Tarasov Q, Quintero T, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a stakeholder-survey study". Euphytica 46(2023): 289-296. DOI: 10.3389/fdgth.2026.1763884

Urbanek Z, Zielinski U, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a field trial". Front Genome Ed 8(2014): 300-308. DOI: 10.2196/48297

Vasquez A, Alvez V, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a systematic review". CRISPR J 10(2017): 311-320. DOI: 10.1007/s00330-025-12031-z

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Andric V, Vasquez A, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a multi-generation study". Plant Biotechnol J 16(2014): 344-356. DOI: 10.2196/69678

Balogh E, Emmerich B, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a pilot deployment". Theor Appl Genet 18(2017): 355-368. DOI: 10.7759/cureus.46454

Chiriac F, Farkas C, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a case study". Front Plant Sci 20(2020): 366-380. DOI: 10.1016/j.ssci.2025.107028

Doncheva M, Marchetti D, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a meta-analysis". Transgenic Res 22(2023): 377-383. DOI: 10.2196/48633

Emmerich T, Tarasov E, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a comparative trial". GM Crops Food 24(2014): 388-395. DOI: 10.1371/journal.pone.0305949

Foscarini C, Chiriac F, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a stakeholder-survey study". Plant Cell Rep 26(2017): 399-407. DOI: 10.1371/journal.pdig.0000514

Alvez D, Dumitrescu A, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a field trial". Mol Plant 28(2020): 410-419. DOI: 10.17219/acem/208596

Brankovic K, Kowalczyk B, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a systematic review". J Exp Bot 30(2023): 421-431. DOI: 10.7759/cureus.104086

Castellani R, Ristic C, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a confined-trial evaluation". Crop Sci 32(2014): 432-443. DOI: 10.1038/s41746-023-00852-5

Dumitrescu A, Andric D, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a benchmark comparison". Agron J 34(2017): 443-455. DOI: 10.1016/j.mayocpiqo.2022.09.004

Eriksdottir B, Brankovic E, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a multi-generation study". Plant Physiol 36(2020): 454-467. DOI: 10.1177/08404704241291226

Farkas I, Ionescu F, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a pilot deployment". New Phytol 38(2023): 465-479. DOI: 10.1093/medlaw/fwad013

Grabowski P, Petrescu G, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a case study". Nat Biotechnol 40(2014): 476-482. DOI: 10.2196/89834

Hendriksen W, Wojcik H, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a meta-analysis". Sci Rep 42(2017): 487-494. DOI: 10.1007/s10916-024-02104-9

Ionescu F, Foscarini I, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a comparative trial". PLoS One 44(2020): 498-506. DOI: 10.3389/fdgth.2026.1896184

Jankowiak G, Grabowski J, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a stakeholder-survey study". BMC Plant Biol 46(2023): 509-518. DOI: 10.3390/diagnostics15162117

Kowalczyk N, Novotna K, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a field trial". Biotechnol Adv 8(2014): 520-530. DOI: 10.4103/jrpp.jrpp_20_65

Lindqvist U, Urbanek L, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a systematic review". Plant Mol Biol 10(2017): 531-542. DOI: 10.3389/fdgth.2026.1763884

Marchetti D, Doncheva M, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a confined-trial evaluation". Field Crops Res 12(2020): 542-554. DOI: 10.2196/48297

Novotna E, Eriksdottir N, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a benchmark comparison". Euphytica 14(2023): 553-566. DOI: 10.1007/s00330-025-12031-z

Ostrowski L, Lindqvist O, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a multi-generation study". Front Genome Ed 16(2014): 564-578. DOI: 10.3389/fdgth.2026.1699005

Petrescu S, Szabo P, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a pilot deployment". CRISPR J 18(2017): 575-581. DOI: 10.3389/fdgth.2026.1834800

Quintero B, Balogh Q, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a case study". Plant Cell 20(2020): 586-593. DOI: 10.2196/69678

Ristic C, Castellani R, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a meta-analysis". Trends Biotechnol 22(2023): 597-605. DOI: 10.7759/cureus.46454

Szabo J, Jankowiak S, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a comparative trial". Plant Biotechnol J 24(2014): 608-617. DOI: 10.1016/j.ssci.2025.107028

Tarasov Q, Quintero T, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a stakeholder-survey study". Theor Appl Genet 26(2017): 619-629. DOI: 10.2196/48633

Urbanek Z, Zielinski U, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a field trial". Front Plant Sci 28(2020): 630-641. DOI: 10.1371/journal.pone.0305949

Vasquez A, Alvez V, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a systematic review". Transgenic Res 30(2023): 641-653. DOI: 10.1371/journal.pdig.0000514

Wojcik H, Hendriksen W, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a confined-trial evaluation". GM Crops Food 32(2014): 652-665. DOI: 10.17219/acem/208596

Zielinski O, Ostrowski Z, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a benchmark comparison". Plant Cell Rep 34(2017): 663-677. DOI: 10.7759/cureus.104086

Andric V, Vasquez A, et al. "Virus-Induced Gene Silencing for Functional Screening in Legumes: a multi-generation study". Mol Plant 36(2020): 674-680. DOI: 10.1038/s41746-023-00852-5

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Published

2023-03-20

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Vol. 2 No. 1 (2023): vol 2 issue 1 2023

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