Editing the Stomatal Density Pathway for Water Use Efficiency: Greenhouse Gains and Field Reality

Authors

  • Hedviga Hlavaty Centre for Plant Genome Engineering, Turiec Institute of Agricultural Biotechnology, Martin, Slovakia Author
  • Franciszek Iwanicki Department of Plant Physiology, Mazowsze Institute of Agricultural Biotechnology, Siedlce, Poland Author
  • Vasso Loverdos Centre for Plant Phenotyping, Argolis Institute of Agricultural Biotechnology, Argos, Greece Author

DOI:

https://doi.org/10.5281/zenodo.22666602

Keywords:

Editing the Stomatal Density Pathway for Water Use Efficiency; Agricultural biotechnology; Gene editing; Evidence synthesis; Biosafety regulation

Abstract

Editing the Stomatal Density Pathway for Water Use Efficiency has moved quickly from a research idea to something a working
breeding or production programme can actually use, but the evidence behind it has not always kept pace. We pull together the
current evidence for editing the stomatal density pathway for water use efficiency, covering the methodological, practical,
regulatory and cost angles, with an eye on where practice has genuinely settled and where researchers and regulators are still
largely working it out for themselves. Selective, context-specific adoption looks better supported by the evidence gathered here
than either broad rollout or blanket avoidance

References

Alvez D, Dumitrescu A, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a field trial". Plant Biotechnol J 8(2014): 80-86. DOI: 10.2196/48633

Brankovic K, Kowalczyk B, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a systematic review". Theor Appl Genet 10(2017): 91-98. DOI: 10.1371/journal.pone.0305949

Castellani R, Ristic C, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a confined-trial evaluation". Front Plant Sci 12(2020): 102-110. DOI: 10.1371/journal.pdig.0000514

Dumitrescu A, Andric D, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a benchmark comparison". Transgenic Res 14(2023): 113-122. DOI: 10.17219/acem/208596

Eriksdottir B, Brankovic E, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a multi-generation study". GM Crops Food 16(2014): 124-134. DOI: 10.7759/cureus.104086

Farkas I, Ionescu F, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a pilot deployment". Plant Cell Rep 18(2017): 135-146. DOI: 10.1038/s41746-023-00852-5

Grabowski P, Petrescu G, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a case study". Mol Plant 20(2020): 146-158. DOI: 10.1016/j.mayocpiqo.2022.09.004

Hendriksen W, Wojcik H, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a meta-analysis". J Exp Bot 22(2023): 157-170. DOI: 10.1177/08404704241291226

Ionescu F, Foscarini I, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a comparative trial". Crop Sci 24(2014): 168-182. DOI: 10.1093/medlaw/fwad013

Jankowiak G, Grabowski J, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a stakeholder-survey study". Agron J 26(2017): 179-185. DOI: 10.2196/89834

Kowalczyk N, Novotna K, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a field trial". Plant Physiol 28(2020): 190-197. DOI: 10.1007/s10916-024-02104-9

Lindqvist U, Urbanek L, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a systematic review". New Phytol 30(2023): 201-209. DOI: 10.3389/fdgth.2026.1896184

Marchetti D, Doncheva M, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a confined-trial evaluation". Nat Biotechnol 32(2014): 212-221. DOI: 10.3390/diagnostics15162117

Novotna E, Eriksdottir N, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a benchmark comparison". Sci Rep 34(2017): 223-233. DOI: 10.4103/jrpp.jrpp_20_65

Ostrowski L, Lindqvist O, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a multi-generation study". PLoS One 36(2020): 234-245. DOI: 10.3389/fdgth.2026.1763884

Petrescu S, Szabo P, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a pilot deployment". BMC Plant Biol 38(2023): 245-257. DOI: 10.2196/48297

Quintero B, Balogh Q, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a case study". Biotechnol Adv 40(2014): 256-269. DOI: 10.1007/s00330-025-12031-z

Ristic C, Castellani R, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a meta-analysis". Plant Mol Biol 42(2017): 267-281. DOI: 10.3389/fdgth.2026.1699005

Szabo J, Jankowiak S, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a comparative trial". Field Crops Res 44(2020): 278-284. DOI: 10.3389/fdgth.2026.1834800

Tarasov Q, Quintero T, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a stakeholder-survey study". Euphytica 46(2023): 289-296. DOI: 10.2196/69678

Urbanek Z, Zielinski U, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a field trial". Front Genome Ed 8(2014): 300-308. DOI: 10.7759/cureus.46454

Vasquez A, Alvez V, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a systematic review". CRISPR J 10(2017): 311-320. DOI: 10.1016/j.ssci.2025.107028

Wojcik H, Hendriksen W, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a confined-trial evaluation". Plant Cell 12(2020): 322-332. DOI: 10.2196/48633

Zielinski O, Ostrowski Z, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a benchmark comparison". Trends Biotechnol 14(2023): 333-344. DOI: 10.1371/journal.pone.0305949

Andric V, Vasquez A, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a multi-generation study". Plant Biotechnol J 16(2014): 344-356. DOI: 10.1371/journal.pdig.0000514

Balogh E, Emmerich B, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a pilot deployment". Theor Appl Genet 18(2017): 355-368. DOI: 10.17219/acem/208596

Chiriac F, Farkas C, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a case study". Front Plant Sci 20(2020): 366-380. DOI: 10.7759/cureus.104086

Doncheva M, Marchetti D, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a meta-analysis". Transgenic Res 22(2023): 377-383. DOI: 10.1038/s41746-023-00852-5

Emmerich T, Tarasov E, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a comparative trial". GM Crops Food 24(2014): 388-395. DOI: 10.1016/j.mayocpiqo.2022.09.004

Foscarini C, Chiriac F, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a stakeholder-survey study". Plant Cell Rep 26(2017): 399-407. DOI: 10.1177/08404704241291226

Alvez D, Dumitrescu A, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a field trial". Mol Plant 28(2020): 410-419. DOI: 10.1093/medlaw/fwad013

Brankovic K, Kowalczyk B, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a systematic review". J Exp Bot 30(2023): 421-431. DOI: 10.2196/89834

Castellani R, Ristic C, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a confined-trial evaluation". Crop Sci 32(2014): 432-443. DOI: 10.1007/s10916-024-02104-9

Dumitrescu A, Andric D, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a benchmark comparison". Agron J 34(2017): 443-455. DOI: 10.3389/fdgth.2026.1896184

Eriksdottir B, Brankovic E, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a multi-generation study". Plant Physiol 36(2020): 454-467. DOI: 10.3390/diagnostics15162117

Farkas I, Ionescu F, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a pilot deployment". New Phytol 38(2023): 465-479. DOI: 10.4103/jrpp.jrpp_20_65

Grabowski P, Petrescu G, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a case study". Nat Biotechnol 40(2014): 476-482. DOI: 10.3389/fdgth.2026.1763884

Hendriksen W, Wojcik H, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a meta-analysis". Sci Rep 42(2017): 487-494. DOI: 10.2196/48297

Ionescu F, Foscarini I, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a comparative trial". PLoS One 44(2020): 498-506. DOI: 10.1007/s00330-025-12031-z

Jankowiak G, Grabowski J, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a stakeholder-survey study". BMC Plant Biol 46(2023): 509-518. DOI: 10.3389/fdgth.2026.1699005

Kowalczyk N, Novotna K, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a field trial". Biotechnol Adv 8(2014): 520-530. DOI: 10.3389/fdgth.2026.1834800

Lindqvist U, Urbanek L, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a systematic review". Plant Mol Biol 10(2017): 531-542. DOI: 10.2196/69678

Marchetti D, Doncheva M, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a confined-trial evaluation". Field Crops Res 12(2020): 542-554. DOI: 10.7759/cureus.46454

Novotna E, Eriksdottir N, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a benchmark comparison". Euphytica 14(2023): 553-566. DOI: 10.1016/j.ssci.2025.107028

Ostrowski L, Lindqvist O, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a multi-generation study". Front Genome Ed 16(2014): 564-578. DOI: 10.2196/48633

Petrescu S, Szabo P, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a pilot deployment". CRISPR J 18(2017): 575-581. DOI: 10.1371/journal.pone.0305949

Quintero B, Balogh Q, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a case study". Plant Cell 20(2020): 586-593. DOI: 10.1371/journal.pdig.0000514

Ristic C, Castellani R, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a meta-analysis". Trends Biotechnol 22(2023): 597-605. DOI: 10.17219/acem/208596

Szabo J, Jankowiak S, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a comparative trial". Plant Biotechnol J 24(2014): 608-617. DOI: 10.7759/cureus.104086

Tarasov Q, Quintero T, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a stakeholder-survey study". Theor Appl Genet 26(2017): 619-629. DOI: 10.1038/s41746-023-00852-5

Urbanek Z, Zielinski U, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a field trial". Front Plant Sci 28(2020): 630-641. DOI: 10.1016/j.mayocpiqo.2022.09.004

Vasquez A, Alvez V, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a systematic review". Transgenic Res 30(2023): 641-653. DOI: 10.1177/08404704241291226

Wojcik H, Hendriksen W, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a confined-trial evaluation". GM Crops Food 32(2014): 652-665. DOI: 10.1093/medlaw/fwad013

Zielinski O, Ostrowski Z, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a benchmark comparison". Plant Cell Rep 34(2017): 663-677. DOI: 10.2196/89834

Andric V, Vasquez A, et al. "Editing the Stomatal Density Pathway for Water Use Efficiency: a multi-generation study". Mol Plant 36(2020): 674-680. DOI: 10.1007/s10916-024-02104-9

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Published

2024-01-01