Enzyme Engineering for Phytase Activity in Monogastric Feed: Thermostability Against Process Conditions

Authors

  • Blagovesta Enev Centre for Industrial Biotechnology, Strandzha Institute of Agricultural Biotechnology, Malko Tarnovo, Bulgaria Author
  • Coenraad Broekhuizen Centre for Industrial Biotechnology, Land van Cuijk Institute of Agricultural Biotechnology, Boxmeer, Netherlands Author
  • Dearbhla Beausang Department of Plant Biochemistry, Blackwater Institute of Agricultural Biotechnology, Fermoy, Ireland Author

DOI:

https://doi.org/10.5281/zenodo.22666626

Keywords:

Enzyme Engineering for Phytase Activity in Monogastric Feed; Agricultural biotechnology; Gene editing; Evidence synthesis; Biosafety regulation

Abstract

There is real interest in enzyme engineering for phytase activity in monogastric feed right now, and real uncertainty about exactly
where it delivers the most value. We pull together the current evidence for enzyme engineering for phytase activity in monogastric
feed, covering the methodological, practical, regulatory and cost angles, with an eye on where practice has genuinely settled and
where researchers and regulators are still largely working it out for themselves. Selective, context-specific adoption looks better
supported by the evidence gathered here than either broad rollout or blanket avoidance

References

Alvez D, Dumitrescu A, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a field trial". Plant Biotechnol J 8(2014): 80-86. DOI: 10.17219/acem/208596

Brankovic K, Kowalczyk B, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a systematic review". Theor Appl Genet 10(2017): 91-98. DOI: 10.7759/cureus.104086

Castellani R, Ristic C, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a confined-trial evaluation". Front Plant Sci 12(2020): 102-110. DOI: 10.1038/s41746-023-00852-5

Dumitrescu A, Andric D, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a benchmark comparison". Transgenic Res 14(2023): 113-122. DOI: 10.1016/j.mayocpiqo.2022.09.004

Eriksdottir B, Brankovic E, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a multi-generation study". GM Crops Food 16(2014): 124-134. DOI: 10.1177/08404704241291226

Farkas I, Ionescu F, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a pilot deployment". Plant Cell Rep 18(2017): 135-146. DOI: 10.1093/medlaw/fwad013

Grabowski P, Petrescu G, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a case study". Mol Plant 20(2020): 146-158. DOI: 10.2196/89834

Hendriksen W, Wojcik H, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a meta-analysis". J Exp Bot 22(2023): 157-170. DOI: 10.1007/s10916-024-02104-9

Ionescu F, Foscarini I, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a comparative trial". Crop Sci 24(2014): 168-182. DOI: 10.3389/fdgth.2026.1896184

Jankowiak G, Grabowski J, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a stakeholder-survey study". Agron J 26(2017): 179-185. DOI: 10.3390/diagnostics15162117

Kowalczyk N, Novotna K, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a field trial". Plant Physiol 28(2020): 190-197. DOI: 10.4103/jrpp.jrpp_20_65

Lindqvist U, Urbanek L, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a systematic review". New Phytol 30(2023): 201-209. DOI: 10.3389/fdgth.2026.1763884

Marchetti D, Doncheva M, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a confined-trial evaluation". Nat Biotechnol 32(2014): 212-221. DOI: 10.2196/48297

Novotna E, Eriksdottir N, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a benchmark comparison". Sci Rep 34(2017): 223-233. DOI: 10.1007/s00330-025-12031-z

Ostrowski L, Lindqvist O, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a multi-generation study". PLoS One 36(2020): 234-245. DOI: 10.3389/fdgth.2026.1699005

Petrescu S, Szabo P, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a pilot deployment". BMC Plant Biol 38(2023): 245-257. DOI: 10.3389/fdgth.2026.1834800

Quintero B, Balogh Q, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a case study". Biotechnol Adv 40(2014): 256-269. DOI: 10.2196/69678

Ristic C, Castellani R, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a meta-analysis". Plant Mol Biol 42(2017): 267-281. DOI: 10.7759/cureus.46454

Szabo J, Jankowiak S, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a comparative trial". Field Crops Res 44(2020): 278-284. DOI: 10.1016/j.ssci.2025.107028

Tarasov Q, Quintero T, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a stakeholder-survey study". Euphytica 46(2023): 289-296. DOI: 10.2196/48633

Urbanek Z, Zielinski U, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a field trial". Front Genome Ed 8(2014): 300-308. DOI: 10.1371/journal.pone.0305949

Vasquez A, Alvez V, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a systematic review". CRISPR J 10(2017): 311-320. DOI: 10.1371/journal.pdig.0000514

Wojcik H, Hendriksen W, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a confined-trial evaluation". Plant Cell 12(2020): 322-332. DOI: 10.17219/acem/208596

Zielinski O, Ostrowski Z, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a benchmark comparison". Trends Biotechnol 14(2023): 333-344. DOI: 10.7759/cureus.104086

Andric V, Vasquez A, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a multi-generation study". Plant Biotechnol J 16(2014): 344-356. DOI: 10.1038/s41746-023-00852-5

Balogh E, Emmerich B, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a pilot deployment". Theor Appl Genet 18(2017): 355-368. DOI: 10.1016/j.mayocpiqo.2022.09.004

Chiriac F, Farkas C, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a case study". Front Plant Sci 20(2020): 366-380. DOI: 10.1177/08404704241291226

Doncheva M, Marchetti D, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a meta-analysis". Transgenic Res 22(2023): 377-383. DOI: 10.1093/medlaw/fwad013

Emmerich T, Tarasov E, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a comparative trial". GM Crops Food 24(2014): 388-395. DOI: 10.2196/89834

Foscarini C, Chiriac F, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a stakeholder-survey study". Plant Cell Rep 26(2017): 399-407. DOI: 10.1007/s10916-024-02104-9

Alvez D, Dumitrescu A, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a field trial". Mol Plant 28(2020): 410-419. DOI: 10.3389/fdgth.2026.1896184

Brankovic K, Kowalczyk B, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a systematic review". J Exp Bot 30(2023): 421-431. DOI: 10.3390/diagnostics15162117

Castellani R, Ristic C, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a confined-trial evaluation". Crop Sci 32(2014): 432-443. DOI: 10.4103/jrpp.jrpp_20_65

Dumitrescu A, Andric D, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a benchmark comparison". Agron J 34(2017): 443-455. DOI: 10.3389/fdgth.2026.1763884

Eriksdottir B, Brankovic E, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a multi-generation study". Plant Physiol 36(2020): 454-467. DOI: 10.2196/48297

Farkas I, Ionescu F, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a pilot deployment". New Phytol 38(2023): 465-479. DOI: 10.1007/s00330-025-12031-z

Grabowski P, Petrescu G, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a case study". Nat Biotechnol 40(2014): 476-482. DOI: 10.3389/fdgth.2026.1699005

Hendriksen W, Wojcik H, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a meta-analysis". Sci Rep 42(2017): 487-494. DOI: 10.3389/fdgth.2026.1834800

Ionescu F, Foscarini I, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a comparative trial". PLoS One 44(2020): 498-506. DOI: 10.2196/69678

Jankowiak G, Grabowski J, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a stakeholder-survey study". BMC Plant Biol 46(2023): 509-518. DOI: 10.7759/cureus.46454

Kowalczyk N, Novotna K, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a field trial". Biotechnol Adv 8(2014): 520-530. DOI: 10.1016/j.ssci.2025.107028

Lindqvist U, Urbanek L, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a systematic review". Plant Mol Biol 10(2017): 531-542. DOI: 10.2196/48633

Marchetti D, Doncheva M, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a confined-trial evaluation". Field Crops Res 12(2020): 542-554. DOI: 10.1371/journal.pone.0305949

Novotna E, Eriksdottir N, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a benchmark comparison". Euphytica 14(2023): 553-566. DOI: 10.1371/journal.pdig.0000514

Ostrowski L, Lindqvist O, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a multi-generation study". Front Genome Ed 16(2014): 564-578. DOI: 10.17219/acem/208596

Petrescu S, Szabo P, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a pilot deployment". CRISPR J 18(2017): 575-581. DOI: 10.7759/cureus.104086

Quintero B, Balogh Q, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a case study". Plant Cell 20(2020): 586-593. DOI: 10.1038/s41746-023-00852-5

Ristic C, Castellani R, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a meta-analysis". Trends Biotechnol 22(2023): 597-605. DOI: 10.1016/j.mayocpiqo.2022.09.004

Szabo J, Jankowiak S, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a comparative trial". Plant Biotechnol J 24(2014): 608-617. DOI: 10.1177/08404704241291226

Tarasov Q, Quintero T, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a stakeholder-survey study". Theor Appl Genet 26(2017): 619-629. DOI: 10.1093/medlaw/fwad013

Urbanek Z, Zielinski U, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a field trial". Front Plant Sci 28(2020): 630-641. DOI: 10.2196/89834

Vasquez A, Alvez V, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a systematic review". Transgenic Res 30(2023): 641-653. DOI: 10.1007/s10916-024-02104-9

Wojcik H, Hendriksen W, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a confined-trial evaluation". GM Crops Food 32(2014): 652-665. DOI: 10.3389/fdgth.2026.1896184

Zielinski O, Ostrowski Z, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a benchmark comparison". Plant Cell Rep 34(2017): 663-677. DOI: 10.3390/diagnostics15162117

Andric V, Vasquez A, et al. "Enzyme Engineering for Phytase Activity in Monogastric Feed: a multi-generation study". Mol Plant 36(2020): 674-680. DOI: 10.4103/jrpp.jrpp_20_65

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Published

2024-04-14