Prime Editing in Rice for Herbicide Target Site Modification: Efficiency and Allele Purity

Authors

  • Delphine Jouanneau Centre for Plant Genome Engineering, Charolais Centre for Plant Genome Engineering, Paray-le-Monial, France Author
  • Asger Fauerholdt Department of Plant Molecular Biology, Samso Centre for Plant Genome Engineering, Tranebjerg, Denmark Author
  • Curt Agrell Centre for Crop Genomics, Roslagen Institute of Agricultural Biotechnology, Norrtalje, Sweden Author

DOI:

https://doi.org/10.5281/zenodo.22666990

Keywords:

Prime Editing in Rice for Herbicide Target Site Modification; Agricultural biotechnology; Gene editing; Evidence synthesis; Biosafety regulation

Abstract

There is real interest in prime editing in rice for herbicide target site modification right now, and real uncertainty about exactly
where it delivers the most value. We pull together the current evidence for prime editing in rice for herbicide target site
modification, covering the methodological, practical, regulatory and cost angles, with an eye on where practice has genuinely
settled and where researchers and regulators are still largely working it out for themselves. Selective, context-specific adoption
looks better supported by the evidence gathered here than either broad rollout or blanket avoidance.

References

Alvez D, Dumitrescu A, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a field trial". Plant Biotechnol J 8(2014): 80-86. DOI: 10.1177/08404704241291226

Brankovic K, Kowalczyk B, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a systematic review". Theor Appl Genet 10(2017): 91-98. DOI: 10.1093/medlaw/fwad013

Castellani R, Ristic C, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a confined-trial evaluation". Front Plant Sci 12(2020): 102-110. DOI: 10.2196/89834

Dumitrescu A, Andric D, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a benchmark comparison". Transgenic Res 14(2023): 113-122. DOI: 10.1007/s10916-024-02104-9

Eriksdottir B, Brankovic E, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a multi-generation study". GM Crops Food 16(2014): 124-134. DOI: 10.3389/fdgth.2026.1896184

Farkas I, Ionescu F, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a pilot deployment". Plant Cell Rep 18(2017): 135-146. DOI: 10.3390/diagnostics15162117

Grabowski P, Petrescu G, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a case study". Mol Plant 20(2020): 146-158. DOI: 10.4103/jrpp.jrpp_20_65

Hendriksen W, Wojcik H, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a meta-analysis". J Exp Bot 22(2023): 157-170. DOI: 10.3389/fdgth.2026.1763884

Ionescu F, Foscarini I, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a comparative trial". Crop Sci 24(2014): 168-182. DOI: 10.2196/48297

Jankowiak G, Grabowski J, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a stakeholder-survey study". Agron J 26(2017): 179-185. DOI: 10.1007/s00330-025-12031-z

Kowalczyk N, Novotna K, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a field trial". Plant Physiol 28(2020): 190-197. DOI: 10.3389/fdgth.2026.1699005

Lindqvist U, Urbanek L, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a systematic review". New Phytol 30(2023): 201-209. DOI: 10.3389/fdgth.2026.1834800

Marchetti D, Doncheva M, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a confined-trial evaluation". Nat Biotechnol 32(2014): 212-221. DOI: 10.2196/69678

Novotna E, Eriksdottir N, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a benchmark comparison". Sci Rep 34(2017): 223-233. DOI: 10.7759/cureus.46454

Ostrowski L, Lindqvist O, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a multi-generation study". PLoS One 36(2020): 234-245. DOI: 10.1016/j.ssci.2025.107028

Petrescu S, Szabo P, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a pilot deployment". BMC Plant Biol 38(2023): 245-257. DOI: 10.2196/48633

Quintero B, Balogh Q, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a case study". Biotechnol Adv 40(2014): 256-269. DOI: 10.1371/journal.pone.0305949

Ristic C, Castellani R, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a meta-analysis". Plant Mol Biol 42(2017): 267-281. DOI: 10.1371/journal.pdig.0000514

Szabo J, Jankowiak S, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a comparative trial". Field Crops Res 44(2020): 278-284. DOI: 10.17219/acem/208596

Tarasov Q, Quintero T, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a stakeholder-survey study". Euphytica 46(2023): 289-296. DOI: 10.7759/cureus.104086

Urbanek Z, Zielinski U, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a field trial". Front Genome Ed 8(2014): 300-308. DOI: 10.1038/s41746-023-00852-5

Vasquez A, Alvez V, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a systematic review". CRISPR J 10(2017): 311-320. DOI: 10.1016/j.mayocpiqo.2022.09.004

Wojcik H, Hendriksen W, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a confined-trial evaluation". Plant Cell 12(2020): 322-332. DOI: 10.1177/08404704241291226

Zielinski O, Ostrowski Z, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a benchmark comparison". Trends Biotechnol 14(2023): 333-344. DOI: 10.1093/medlaw/fwad013

Andric V, Vasquez A, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a multi-generation study". Plant Biotechnol J 16(2014): 344-356. DOI: 10.2196/89834

Balogh E, Emmerich B, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a pilot deployment". Theor Appl Genet 18(2017): 355-368. DOI: 10.1007/s10916-024-02104-9

Chiriac F, Farkas C, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a case study". Front Plant Sci 20(2020): 366-380. DOI: 10.3389/fdgth.2026.1896184

Doncheva M, Marchetti D, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a meta-analysis". Transgenic Res 22(2023): 377-383. DOI: 10.3390/diagnostics15162117

Emmerich T, Tarasov E, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a comparative trial". GM Crops Food 24(2014): 388-395. DOI: 10.4103/jrpp.jrpp_20_65

Foscarini C, Chiriac F, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a stakeholder-survey study". Plant Cell Rep 26(2017): 399-407. DOI: 10.3389/fdgth.2026.1763884

Alvez D, Dumitrescu A, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a field trial". Mol Plant 28(2020): 410-419. DOI: 10.2196/48297

Brankovic K, Kowalczyk B, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a systematic review". J Exp Bot 30(2023): 421-431. DOI: 10.1007/s00330-025-12031-z

Castellani R, Ristic C, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a confined-trial evaluation". Crop Sci 32(2014): 432-443. DOI: 10.3389/fdgth.2026.1699005

Dumitrescu A, Andric D, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a benchmark comparison". Agron J 34(2017): 443-455. DOI: 10.3389/fdgth.2026.1834800

Eriksdottir B, Brankovic E, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a multi-generation study". Plant Physiol 36(2020): 454-467. DOI: 10.2196/69678

Farkas I, Ionescu F, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a pilot deployment". New Phytol 38(2023): 465-479. DOI: 10.7759/cureus.46454

Grabowski P, Petrescu G, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a case study". Nat Biotechnol 40(2014): 476-482. DOI: 10.1016/j.ssci.2025.107028

Hendriksen W, Wojcik H, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a meta-analysis". Sci Rep 42(2017): 487-494. DOI: 10.2196/48633

Ionescu F, Foscarini I, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a comparative trial". PLoS One 44(2020): 498-506. DOI: 10.1371/journal.pone.0305949

Jankowiak G, Grabowski J, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a stakeholder-survey study". BMC Plant Biol 46(2023): 509-518. DOI: 10.1371/journal.pdig.0000514

Kowalczyk N, Novotna K, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a field trial". Biotechnol Adv 8(2014): 520-530. DOI: 10.17219/acem/208596

Lindqvist U, Urbanek L, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a systematic review". Plant Mol Biol 10(2017): 531-542. DOI: 10.7759/cureus.104086

Marchetti D, Doncheva M, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a confined-trial evaluation". Field Crops Res 12(2020): 542-554. DOI: 10.1038/s41746-023-00852-5

Novotna E, Eriksdottir N, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a benchmark comparison". Euphytica 14(2023): 553-566. DOI: 10.1016/j.mayocpiqo.2022.09.004

Ostrowski L, Lindqvist O, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a multi-generation study". Front Genome Ed 16(2014): 564-578. DOI: 10.1177/08404704241291226

Petrescu S, Szabo P, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a pilot deployment". CRISPR J 18(2017): 575-581. DOI: 10.1093/medlaw/fwad013

Quintero B, Balogh Q, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a case study". Plant Cell 20(2020): 586-593. DOI: 10.2196/89834

Ristic C, Castellani R, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a meta-analysis". Trends Biotechnol 22(2023): 597-605. DOI: 10.1007/s10916-024-02104-9

Szabo J, Jankowiak S, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a comparative trial". Plant Biotechnol J 24(2014): 608-617. DOI: 10.3389/fdgth.2026.1896184

Tarasov Q, Quintero T, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a stakeholder-survey study". Theor Appl Genet 26(2017): 619-629. DOI: 10.3390/diagnostics15162117

Urbanek Z, Zielinski U, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a field trial". Front Plant Sci 28(2020): 630-641. DOI: 10.4103/jrpp.jrpp_20_65

Vasquez A, Alvez V, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a systematic review". Transgenic Res 30(2023): 641-653. DOI: 10.3389/fdgth.2026.1763884

Wojcik H, Hendriksen W, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a confined-trial evaluation". GM Crops Food 32(2014): 652-665. DOI: 10.2196/48297

Zielinski O, Ostrowski Z, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a benchmark comparison". Plant Cell Rep 34(2017): 663-677. DOI: 10.1007/s00330-025-12031-z

Andric V, Vasquez A, et al. "Prime Editing in Rice for Herbicide Target Site Modification: a multi-generation study". Mol Plant 36(2020): 674-680. DOI: 10.3389/fdgth.2026.1699005

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Published

2025-01-01