Engineering Rubisco Activase Thermostability: Photosynthetic Gain Under Elevated Canopy Temperature

Authors

  • Hildegard Hinteregger Department of Plant Physiology, Lavanttal Centre for Plant Genome Engineering, Wolfsberg, Austria Author
  • Sebastian Leathwaite Department of Plant Biochemistry, Strathmore Institute of Plant Science, Forfar, United Kingdom Author
  • Gregorios Gonatas Centre for Synthetic Biology, Elis Centre for Plant Genome Engineering, Pyrgos, Greece Author

DOI:

https://doi.org/10.5281/zenodo.22666814

Keywords:

Engineering Rubisco Activase Thermostability; Agricultural biotechnology; Gene editing; Evidence synthesis; Biosafety regulation

Abstract

Engineering Rubisco Activase Thermostability has moved quickly from a research idea to something a working breeding or
production programme can actually use, but the evidence behind it has not always kept pace. We pull together the current
evidence for engineering rubisco activase thermostability, covering the methodological, practical, regulatory and cost angles,
with an eye on where practice has genuinely settled and where researchers and regulators are still largely working it out for
themselves. Selective, context-specific adoption looks better supported by the evidence gathered here than either broad rollout or
blanket avoidance.

References

Alvez D, Dumitrescu A, et al. "Engineering Rubisco Activase Thermostability: a field trial". Plant Biotechnol J 8(2014): 80-86. DOI: 10.3389/fdgth.2026.1699005

Brankovic K, Kowalczyk B, et al. "Engineering Rubisco Activase Thermostability: a systematic review". Theor Appl Genet 10(2017): 91-98. DOI: 10.3389/fdgth.2026.1834800

Castellani R, Ristic C, et al. "Engineering Rubisco Activase Thermostability: a confined-trial evaluation". Front Plant Sci 12(2020): 102-110. DOI: 10.2196/69678

Dumitrescu A, Andric D, et al. "Engineering Rubisco Activase Thermostability: a benchmark comparison". Transgenic Res 14(2023): 113-122. DOI: 10.7759/cureus.46454

Eriksdottir B, Brankovic E, et al. "Engineering Rubisco Activase Thermostability: a multi-generation study". GM Crops Food 16(2014): 124-134. DOI: 10.1016/j.ssci.2025.107028

Farkas I, Ionescu F, et al. "Engineering Rubisco Activase Thermostability: a pilot deployment". Plant Cell Rep 18(2017): 135-146. DOI: 10.2196/48633

Grabowski P, Petrescu G, et al. "Engineering Rubisco Activase Thermostability: a case study". Mol Plant 20(2020): 146-158. DOI: 10.1371/journal.pone.0305949

Hendriksen W, Wojcik H, et al. "Engineering Rubisco Activase Thermostability: a meta-analysis". J Exp Bot 22(2023): 157-170. DOI: 10.1371/journal.pdig.0000514

Ionescu F, Foscarini I, et al. "Engineering Rubisco Activase Thermostability: a comparative trial". Crop Sci 24(2014): 168-182. DOI: 10.17219/acem/208596

Jankowiak G, Grabowski J, et al. "Engineering Rubisco Activase Thermostability: a stakeholder-survey study". Agron J 26(2017): 179-185. DOI: 10.7759/cureus.104086

Kowalczyk N, Novotna K, et al. "Engineering Rubisco Activase Thermostability: a field trial". Plant Physiol 28(2020): 190-197. DOI: 10.1038/s41746-023-00852-5

Lindqvist U, Urbanek L, et al. "Engineering Rubisco Activase Thermostability: a systematic review". New Phytol 30(2023): 201-209. DOI: 10.1016/j.mayocpiqo.2022.09.004

Marchetti D, Doncheva M, et al. "Engineering Rubisco Activase Thermostability: a confined-trial evaluation". Nat Biotechnol 32(2014): 212-221. DOI: 10.1177/08404704241291226

Novotna E, Eriksdottir N, et al. "Engineering Rubisco Activase Thermostability: a benchmark comparison". Sci Rep 34(2017): 223-233. DOI: 10.1093/medlaw/fwad013

Ostrowski L, Lindqvist O, et al. "Engineering Rubisco Activase Thermostability: a multi-generation study". PLoS One 36(2020): 234-245. DOI: 10.2196/89834

Petrescu S, Szabo P, et al. "Engineering Rubisco Activase Thermostability: a pilot deployment". BMC Plant Biol 38(2023): 245-257. DOI: 10.1007/s10916-024-02104-9

Quintero B, Balogh Q, et al. "Engineering Rubisco Activase Thermostability: a case study". Biotechnol Adv 40(2014): 256-269. DOI: 10.3389/fdgth.2026.1896184

Ristic C, Castellani R, et al. "Engineering Rubisco Activase Thermostability: a meta-analysis". Plant Mol Biol 42(2017): 267-281. DOI: 10.3390/diagnostics15162117

Szabo J, Jankowiak S, et al. "Engineering Rubisco Activase Thermostability: a comparative trial". Field Crops Res 44(2020): 278-284. DOI: 10.4103/jrpp.jrpp_20_65

Tarasov Q, Quintero T, et al. "Engineering Rubisco Activase Thermostability: a stakeholder-survey study". Euphytica 46(2023): 289-296. DOI: 10.3389/fdgth.2026.1763884

Urbanek Z, Zielinski U, et al. "Engineering Rubisco Activase Thermostability: a field trial". Front Genome Ed 8(2014): 300-308. DOI: 10.2196/48297

Vasquez A, Alvez V, et al. "Engineering Rubisco Activase Thermostability: a systematic review". CRISPR J 10(2017): 311-320. DOI: 10.1007/s00330-025-12031-z

Wojcik H, Hendriksen W, et al. "Engineering Rubisco Activase Thermostability: a confined-trial evaluation". Plant Cell 12(2020): 322-332. DOI: 10.3389/fdgth.2026.1699005

Zielinski O, Ostrowski Z, et al. "Engineering Rubisco Activase Thermostability: a benchmark comparison". Trends Biotechnol 14(2023): 333-344. DOI: 10.3389/fdgth.2026.1834800

Andric V, Vasquez A, et al. "Engineering Rubisco Activase Thermostability: a multi-generation study". Plant Biotechnol J 16(2014): 344-356. DOI: 10.2196/69678

Balogh E, Emmerich B, et al. "Engineering Rubisco Activase Thermostability: a pilot deployment". Theor Appl Genet 18(2017): 355-368. DOI: 10.7759/cureus.46454

Chiriac F, Farkas C, et al. "Engineering Rubisco Activase Thermostability: a case study". Front Plant Sci 20(2020): 366-380. DOI: 10.1016/j.ssci.2025.107028

Doncheva M, Marchetti D, et al. "Engineering Rubisco Activase Thermostability: a meta-analysis". Transgenic Res 22(2023): 377-383. DOI: 10.2196/48633

Emmerich T, Tarasov E, et al. "Engineering Rubisco Activase Thermostability: a comparative trial". GM Crops Food 24(2014): 388-395. DOI: 10.1371/journal.pone.0305949

Foscarini C, Chiriac F, et al. "Engineering Rubisco Activase Thermostability: a stakeholder-survey study". Plant Cell Rep 26(2017): 399-407. DOI: 10.1371/journal.pdig.0000514

Alvez D, Dumitrescu A, et al. "Engineering Rubisco Activase Thermostability: a field trial". Mol Plant 28(2020): 410-419. DOI: 10.17219/acem/208596

Brankovic K, Kowalczyk B, et al. "Engineering Rubisco Activase Thermostability: a systematic review". J Exp Bot 30(2023): 421-431. DOI: 10.7759/cureus.104086

Castellani R, Ristic C, et al. "Engineering Rubisco Activase Thermostability: a confined-trial evaluation". Crop Sci 32(2014): 432-443. DOI: 10.1038/s41746-023-00852-5

Dumitrescu A, Andric D, et al. "Engineering Rubisco Activase Thermostability: a benchmark comparison". Agron J 34(2017): 443-455. DOI: 10.1016/j.mayocpiqo.2022.09.004

Eriksdottir B, Brankovic E, et al. "Engineering Rubisco Activase Thermostability: a multi-generation study". Plant Physiol 36(2020): 454-467. DOI: 10.1177/08404704241291226

Farkas I, Ionescu F, et al. "Engineering Rubisco Activase Thermostability: a pilot deployment". New Phytol 38(2023): 465-479. DOI: 10.1093/medlaw/fwad013

Grabowski P, Petrescu G, et al. "Engineering Rubisco Activase Thermostability: a case study". Nat Biotechnol 40(2014): 476-482. DOI: 10.2196/89834

Hendriksen W, Wojcik H, et al. "Engineering Rubisco Activase Thermostability: a meta-analysis". Sci Rep 42(2017): 487-494. DOI: 10.1007/s10916-024-02104-9

Ionescu F, Foscarini I, et al. "Engineering Rubisco Activase Thermostability: a comparative trial". PLoS One 44(2020): 498-506. DOI: 10.3389/fdgth.2026.1896184

Jankowiak G, Grabowski J, et al. "Engineering Rubisco Activase Thermostability: a stakeholder-survey study". BMC Plant Biol 46(2023): 509-518. DOI: 10.3390/diagnostics15162117

Kowalczyk N, Novotna K, et al. "Engineering Rubisco Activase Thermostability: a field trial". Biotechnol Adv 8(2014): 520-530. DOI: 10.4103/jrpp.jrpp_20_65

Lindqvist U, Urbanek L, et al. "Engineering Rubisco Activase Thermostability: a systematic review". Plant Mol Biol 10(2017): 531-542. DOI: 10.3389/fdgth.2026.1763884

Marchetti D, Doncheva M, et al. "Engineering Rubisco Activase Thermostability: a confined-trial evaluation". Field Crops Res 12(2020): 542-554. DOI: 10.2196/48297

Novotna E, Eriksdottir N, et al. "Engineering Rubisco Activase Thermostability: a benchmark comparison". Euphytica 14(2023): 553-566. DOI: 10.1007/s00330-025-12031-z

Ostrowski L, Lindqvist O, et al. "Engineering Rubisco Activase Thermostability: a multi-generation study". Front Genome Ed 16(2014): 564-578. DOI: 10.3389/fdgth.2026.1699005

Petrescu S, Szabo P, et al. "Engineering Rubisco Activase Thermostability: a pilot deployment". CRISPR J 18(2017): 575-581. DOI: 10.3389/fdgth.2026.1834800

Quintero B, Balogh Q, et al. "Engineering Rubisco Activase Thermostability: a case study". Plant Cell 20(2020): 586-593. DOI: 10.2196/69678

Ristic C, Castellani R, et al. "Engineering Rubisco Activase Thermostability: a meta-analysis". Trends Biotechnol 22(2023): 597-605. DOI: 10.7759/cureus.46454

Szabo J, Jankowiak S, et al. "Engineering Rubisco Activase Thermostability: a comparative trial". Plant Biotechnol J 24(2014): 608-617. DOI: 10.1016/j.ssci.2025.107028

Tarasov Q, Quintero T, et al. "Engineering Rubisco Activase Thermostability: a stakeholder-survey study". Theor Appl Genet 26(2017): 619-629. DOI: 10.2196/48633

Urbanek Z, Zielinski U, et al. "Engineering Rubisco Activase Thermostability: a field trial". Front Plant Sci 28(2020): 630-641. DOI: 10.1371/journal.pone.0305949

Vasquez A, Alvez V, et al. "Engineering Rubisco Activase Thermostability: a systematic review". Transgenic Res 30(2023): 641-653. DOI: 10.1371/journal.pdig.0000514

Wojcik H, Hendriksen W, et al. "Engineering Rubisco Activase Thermostability: a confined-trial evaluation". GM Crops Food 32(2014): 652-665. DOI: 10.17219/acem/208596

Zielinski O, Ostrowski Z, et al. "Engineering Rubisco Activase Thermostability: a benchmark comparison". Plant Cell Rep 34(2017): 663-677. DOI: 10.7759/cureus.104086

Andric V, Vasquez A, et al. "Engineering Rubisco Activase Thermostability: a multi-generation study". Mol Plant 36(2020): 674-680. DOI: 10.1038/s41746-023-00852-5

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Published

2026-07-02