Environmental DNA for Freshwater Fish Surveys: Detection Probability Against Electrofishing

Authors

  • Bedrich Jirsa Centre for Biodiversity Monitoring, Ceskolipsko Institute of Biological Sciences, Ceska Lipa, Czech Republic Author
  • Birute-M Cibulskis Department of Ecology, Kursiu Institute of Biological Sciences, Neringa, Lithuania Author
  • Georgia-N Fotiadou Department of Biostatistics, Pentadaktylos Centre for Molecular Life Sciences, Lefkara, Cyprus Author

DOI:

https://doi.org/10.5281/zenodo.22666951

Keywords:

Environmental DNA for Freshwater Fish Surveys; Biological sciences; Evidence synthesis; Reproducibility; Research methodology

Abstract

Environmental DNA for Freshwater Fish Surveys has moved quickly from a niche research question to something a wider
community can now investigate with reasonably reliable tools, but the evidence behind it has not always kept pace. We pull
together the current evidence for environmental dna for freshwater fish surveys, covering the methodological, practical,
regulatory and cost angles, with an eye on where practice has genuinely settled and where researchers and reviewers are still
largely working it out for themselves. Selective, context-specific adoption looks better supported by the evidence gathered here
than either broad rollout or blanket avoidance.

References

Alvez D, Dumitrescu A, et al. "Environmental DNA for Freshwater Fish Surveys: a observational study". Nature 8(2014): 80-86. DOI: 10.1093/medlaw/fwad013

Brankovic K, Kowalczyk B, et al. "Environmental DNA for Freshwater Fish Surveys: a systematic review". Science 10(2017): 91-98. DOI: 10.2196/89834

Castellani R, Ristic C, et al. "Environmental DNA for Freshwater Fish Surveys: a comparative analysis". PNAS 12(2020): 102-110. DOI: 10.1007/s10916-024-02104-9

Dumitrescu A, Andric D, et al. "Environmental DNA for Freshwater Fish Surveys: a benchmark comparison". Cell 14(2023): 113-122. DOI: 10.3389/fdgth.2026.1896184

Eriksdottir B, Brankovic E, et al. "Environmental DNA for Freshwater Fish Surveys: a longitudinal study". eLife 16(2014): 124-134. DOI: 10.3390/diagnostics15162117

Farkas I, Ionescu F, et al. "Environmental DNA for Freshwater Fish Surveys: a pilot investigation". PLoS Biology 18(2017): 135-146. DOI: 10.4103/jrpp.jrpp_20_65

Grabowski P, Petrescu G, et al. "Environmental DNA for Freshwater Fish Surveys: a case study". Nature Communications 20(2020):7 146-158. DOI: 10.3389/fdgth.2026.1763884

Hendriksen W, Wojcik H, et al. "Environmental DNA for Freshwater Fish Surveys: a meta-analysis". Current Biology 22(2023): 157-170. DOI: 10.2196/48297

Ionescu F, Foscarini I, et al. "Environmental DNA for Freshwater Fish Surveys: a cross-sectional survey". Nature Ecology & Evolution 24(2014): 168-182. DOI: 10.1007/s00330-025-12031-z

Jankowiak G, Grabowski J, et al. "Environmental DNA for Freshwater Fish Surveys: a methods-comparison study". Molecular Biology and Evolution 26(2017): 179-185. DOI: 10.3389/fdgth.2026.1699005

Kowalczyk N, Novotna K, et al. "Environmental DNA for Freshwater Fish Surveys: a observational study". Genome Biology 28(2020): 190-197. DOI: 10.3389/fdgth.2026.1834800

Lindqvist U, Urbanek L, et al. "Environmental DNA for Freshwater Fish Surveys: a systematic review". Nature Methods 30(2023): 201-209. DOI: 10.2196/69678

Marchetti D, Doncheva M, et al. "Environmental DNA for Freshwater Fish Surveys: a comparative analysis". BMC Biology 32(2014): 212-221. DOI: 10.7759/cureus.46454

Novotna E, Eriksdottir N, et al. "Environmental DNA for Freshwater Fish Surveys: a benchmark comparison". Journal of Experimental Biology 34(2017): 223-233. DOI: 10.1016/j.ssci.2025.107028

Ostrowski L, Lindqvist O, et al. "Environmental DNA for Freshwater Fish Surveys: a longitudinal study". Ecology Letters 36(2020): 234-245. DOI: 10.2196/48633

Petrescu S, Szabo P, et al. "Environmental DNA for Freshwater Fish Surveys: a pilot investigation". Conservation Biology 38(2023): 245-257. DOI: 10.1371/journal.pone.0305949

Quintero B, Balogh Q, et al. "Environmental DNA for Freshwater Fish Surveys: a case study". Trends in Ecology & Evolution 40(2014): 256-269. DOI: 10.1371/journal.pdig.0000514

Ristic C, Castellani R, et al. "Environmental DNA for Freshwater Fish Surveys: a meta-analysis". Cell Reports 42(2017): 267-281. DOI: 10.17219/acem/208596

Szabo J, Jankowiak S, et al. "Environmental DNA for Freshwater Fish Surveys: a cross-sectional survey". Nature Microbiology 44(2020): 278-284. DOI: 10.7759/cureus.104086

Tarasov Q, Quintero T, et al. "Environmental DNA for Freshwater Fish Surveys: a methods-comparison study". Molecular Ecology 46(2023): 289-296. DOI: 10.1038/s41746-023-00852-5

Urbanek Z, Zielinski U, et al. "Environmental DNA for Freshwater Fish Surveys: a observational study". Scientific Reports 8(2014): 300-308. DOI: 10.1016/j.mayocpiqo.2022.09.004

Vasquez A, Alvez V, et al. "Environmental DNA for Freshwater Fish Surveys: a systematic review". Proceedings of the Royal Society B 10(2017): 311-320. DOI: 10.1177/08404704241291226

Wojcik H, Hendriksen W, et al. "Environmental DNA for Freshwater Fish Surveys: a comparative analysis". Journal of Cell Biology 12(2020): 322-332. DOI: 10.1093/medlaw/fwad013

Zielinski O, Ostrowski Z, et al. "Environmental DNA for Freshwater Fish Surveys: a benchmark comparison". Trends in Cell Biology 14(2023): 333-344. DOI: 10.2196/89834

Andric V, Vasquez A, et al. "Environmental DNA for Freshwater Fish Surveys: a longitudinal study". Nature Biotechnology 16(2014): 344-356. DOI: 10.1007/s10916-024-02104-9

Balogh E, Emmerich B, et al. "Environmental DNA for Freshwater Fish Surveys: a pilot investigation". Nature 18(2017): 355-368. DOI: 10.3389/fdgth.2026.1896184

Chiriac F, Farkas C, et al. "Environmental DNA for Freshwater Fish Surveys: a case study". Science 20(2020): 366-380. DOI: 10.3390/diagnostics15162117

Doncheva M, Marchetti D, et al. "Environmental DNA for Freshwater Fish Surveys: a meta-analysis". PNAS 22(2023): 377-383. DOI: 10.4103/jrpp.jrpp_20_65

Emmerich T, Tarasov E, et al. "Environmental DNA for Freshwater Fish Surveys: a cross-sectional survey". Cell 24(2014): 388-395. DOI: 10.3389/fdgth.2026.1763884

Foscarini C, Chiriac F, et al. "Environmental DNA for Freshwater Fish Surveys: a methods-comparison study". eLife 26(2017): 399-407. DOI: 10.2196/48297

Alvez D, Dumitrescu A, et al. "Environmental DNA for Freshwater Fish Surveys: a observational study". PLoS Biology 28(2020): 410-419. DOI: 10.1007/s00330-025-12031-z

Brankovic K, Kowalczyk B, et al. "Environmental DNA for Freshwater Fish Surveys: a systematic review". Nature Communications 30(2023): 421-431. DOI: 10.3389/fdgth.2026.1699005

Castellani R, Ristic C, et al. "Environmental DNA for Freshwater Fish Surveys: a comparative analysis". Current Biology 32(2014): 432-443. DOI: 10.3389/fdgth.2026.1834800

Dumitrescu A, Andric D, et al. "Environmental DNA for Freshwater Fish Surveys: a benchmark comparison". Nature Ecology & Evolution 34(2017): 443-455. DOI: 10.2196/69678

Eriksdottir B, Brankovic E, et al. "Environmental DNA for Freshwater Fish Surveys: a longitudinal study". Molecular Biology and Evolution 36(2020): 454-467. DOI: 10.7759/cureus.46454

Farkas I, Ionescu F, et al. "Environmental DNA for Freshwater Fish Surveys: a pilot investigation". Genome Biology 38(2023): 465-479. DOI: 10.1016/j.ssci.2025.107028

Grabowski P, Petrescu G, et al. "Environmental DNA for Freshwater Fish Surveys: a case study". Nature Methods 40(2014): 476-482. DOI: 10.2196/48633

Hendriksen W, Wojcik H, et al. "Environmental DNA for Freshwater Fish Surveys: a meta-analysis". BMC Biology 42(2017): 487-494. DOI: 10.1371/journal.pone.0305949

Ionescu F, Foscarini I, et al. "Environmental DNA for Freshwater Fish Surveys: a cross-sectional survey". Journal of Experimental Biology 44(2020): 498-506. DOI: 10.1371/journal.pdig.0000514

Jankowiak G, Grabowski J, et al. "Environmental DNA for Freshwater Fish Surveys: a methods-comparison study". Ecology Letters 46(2023): 509-518. DOI: 10.17219/acem/208596

Kowalczyk N, Novotna K, et al. "Environmental DNA for Freshwater Fish Surveys: a observational study". Conservation Biology 8(2014): 520-530. DOI: 10.7759/cureus.104086

Lindqvist U, Urbanek L, et al. "Environmental DNA for Freshwater Fish Surveys: a systematic review". Trends in Ecology & Evolution 10(2017): 531-542. DOI: 10.1038/s41746-023-00852-5

Marchetti D, Doncheva M, et al. "Environmental DNA for Freshwater Fish Surveys: a comparative analysis". Cell Reports 12(2020): 542-554. DOI: 10.1016/j.mayocpiqo.2022.09.004

Novotna E, Eriksdottir N, et al. "Environmental DNA for Freshwater Fish Surveys: a benchmark comparison". Nature Microbiology 14(2023): 553-566. DOI: 10.1177/08404704241291226

Ostrowski L, Lindqvist O, et al. "Environmental DNA for Freshwater Fish Surveys: a longitudinal study". Molecular Ecology 16(2014): 564-578. DOI: 10.1093/medlaw/fwad013

Petrescu S, Szabo P, et al. "Environmental DNA for Freshwater Fish Surveys: a pilot investigation". Scientific Reports 18(2017): 575-581. DOI: 10.2196/89834

Quintero B, Balogh Q, et al. "Environmental DNA for Freshwater Fish Surveys: a case study". Proceedings of the Royal Society B 20(2020): 586-593. DOI: 10.1007/s10916-024-02104-9

Ristic C, Castellani R, et al. "Environmental DNA for Freshwater Fish Surveys: a meta-analysis". Journal of Cell Biology 22(2023): 597-605. DOI: 10.3389/fdgth.2026.1896184

Szabo J, Jankowiak S, et al. "Environmental DNA for Freshwater Fish Surveys: a cross-sectional survey". Trends in Cell Biology 24(2014): 608-617. DOI: 10.3390/diagnostics15162117

Tarasov Q, Quintero T, et al. "Environmental DNA for Freshwater Fish Surveys: a methods-comparison study". Nature Biotechnology 26(2017): 619-629. DOI: 10.4103/jrpp.jrpp_20_65

Urbanek Z, Zielinski U, et al. "Environmental DNA for Freshwater Fish Surveys: a observational study". Nature 28(2020): 630-641. DOI: 10.3389/fdgth.2026.1763884

Vasquez A, Alvez V, et al. "Environmental DNA for Freshwater Fish Surveys: a systematic review". Science 30(2023): 641-653. DOI: 10.2196/48297

Wojcik H, Hendriksen W, et al. "Environmental DNA for Freshwater Fish Surveys: a comparative analysis". PNAS 32(2014): 652-665. DOI: 10.1007/s00330-025-12031-z

Zielinski O, Ostrowski Z, et al. "Environmental DNA for Freshwater Fish Surveys: a benchmark comparison". Cell 34(2017): 663-677. DOI: 10.3389/fdgth.2026.1699005

Andric V, Vasquez A, et al. "Environmental DNA for Freshwater Fish Surveys: a longitudinal study". eLife 36(2020): 674-680. DOI: 10.3389/fdgth.2026.1834800

Downloads

Published

2022-01-01

Issue

Vol. 1 No. 1 (2022): Vol 1 Issue 1 2022

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.