Cryopreservation of Non-Model Cell Lines: A Benchmark of Protocols Across Twelve Species

Abstract

There is real interest in cryopreservation of non-model cell lines right now, and real uncertainty about exactly where it delivers
the most value. We pull together the current evidence for cryopreservation of non-model cell lines, covering the methodological,
practical, regulatory and cost angles, with an eye on where practice has genuinely settled and where researchers and reviewers
are still largely working it out for themselves. Selective, context-specific adoption looks better supported by the evidence gathered
here than either broad rollout or blanket avoidance.