Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: A Review of Field Persistence

Authors

  • Into Bergroth Centre for Plant-Microbe Interactions, Pohjois-Savo Institute of Agricultural Biotechnology, Iisalmi, Finland Author
  • Dinos Lambrianides Department of Soil Microbiology, Akamas Institute of Agricultural Biotechnology, Polis, Cyprus Author
  • Julianna-M Cserepes Department of Agronomy, Matyofold Centre for Plant Genome Engineering, Mezokovesd, Hungary Author

DOI:

https://doi.org/10.5281/zenodo.22666292

Keywords:

Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes; Agricultural biotechnology; Gene editing; Evidence synthesis; Biosafety regulation

Abstract

Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes has moved quickly from a research idea to something a working
breeding or production programme can actually use, but the evidence behind it has not always kept pace. We pull together the
current evidence for biofertiliser formulations based on nitrogen-fixing endophytes, covering the methodological, practical,
regulatory and cost angles, with an eye on where practice has genuinely settled and where researchers and regulators are still
largely working it out for themselves. Selective, context-specific adoption looks better supported by the evidence gathered here
than either broad rollout or blanket avoidance.

References

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Brankovic K, Kowalczyk B, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a systematic review". Theor Appl Genet 10(2017): 91-98. DOI: 10.3389/fdgth.2026.1896184

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Dumitrescu A, Andric D, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a benchmark comparison". Transgenic Res 14(2023): 113-122. DOI: 10.4103/jrpp.jrpp_20_65

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Zielinski O, Ostrowski Z, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a benchmark comparison". Trends Biotechnol 14(2023): 333-344. DOI: 10.3389/fdgth.2026.1896184

Andric V, Vasquez A, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a multi-generation study". Plant Biotechnol J 16(2014): 344-356. DOI: 10.3390/diagnostics15162117

Balogh E, Emmerich B, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a pilot deployment". Theor Appl Genet 18(2017): 355-368. DOI: 10.4103/jrpp.jrpp_20_65

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Doncheva M, Marchetti D, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a meta-analysis". Transgenic Res 22(2023): 377-383. DOI: 10.2196/48297

Emmerich T, Tarasov E, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a comparative trial". GM Crops Food 24(2014): 388-395. DOI: 10.1007/s00330-025-12031-z

Foscarini C, Chiriac F, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a stakeholder-survey study". Plant Cell Rep 26(2017): 399-407. DOI: 10.3389/fdgth.2026.1699005

Alvez D, Dumitrescu A, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a field trial". Mol Plant 28(2020): 410-419. DOI: 10.3389/fdgth.2026.1834800

Brankovic K, Kowalczyk B, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a systematic review". J Exp Bot 30(2023): 421-431. DOI: 10.2196/69678

Castellani R, Ristic C, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a confined-trial evaluation". Crop Sci 32(2014): 432-443. DOI: 10.7759/cureus.46454

Dumitrescu A, Andric D, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a benchmark comparison". Agron J 34(2017): 443-455. DOI: 10.1016/j.ssci.2025.107028

Eriksdottir B, Brankovic E, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a multi-generation study". Plant Physiol 36(2020): 454-467. DOI: 10.2196/48633

Farkas I, Ionescu F, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a pilot deployment". New Phytol 38(2023): 465-479. DOI: 10.1371/journal.pone.0305949

Grabowski P, Petrescu G, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a case study". Nat Biotechnol 40(2014): 476-482. DOI: 10.1371/journal.pdig.0000514

Hendriksen W, Wojcik H, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a meta-analysis". Sci Rep 42(2017): 487-494. DOI: 10.17219/acem/208596

Ionescu F, Foscarini I, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a comparative trial". PLoS One 44(2020): 498-506. DOI: 10.7759/cureus.104086

Jankowiak G, Grabowski J, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a stakeholder-survey study". BMC Plant Biol 46(2023): 509-518. DOI: 10.1038/s41746-023-00852-5

Kowalczyk N, Novotna K, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a field trial". Biotechnol Adv 8(2014): 520-530. DOI: 10.1016/j.mayocpiqo.2022.09.004

Lindqvist U, Urbanek L, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a systematic review". Plant Mol Biol 10(2017): 531-542. DOI: 10.1177/08404704241291226

Marchetti D, Doncheva M, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a confined-trial evaluation". Field Crops Res 12(2020): 542-554. DOI: 10.1093/medlaw/fwad013

Novotna E, Eriksdottir N, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a benchmark comparison". Euphytica 14(2023): 553-566. DOI: 10.2196/89834

Ostrowski L, Lindqvist O, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a multi-generation study". Front Genome Ed 16(2014): 564-578. DOI: 10.1007/s10916-024-02104-9

Petrescu S, Szabo P, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a pilot deployment". CRISPR J 18(2017): 575-581. DOI: 10.3389/fdgth.2026.1896184

Quintero B, Balogh Q, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a case study". Plant Cell 20(2020): 586-593. DOI: 10.3390/diagnostics15162117

Ristic C, Castellani R, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a meta-analysis". Trends Biotechnol 22(2023): 597-605. DOI: 10.4103/jrpp.jrpp_20_65

Szabo J, Jankowiak S, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a comparative trial". Plant Biotechnol J 24(2014): 608-617. DOI: 10.3389/fdgth.2026.1763884

Tarasov Q, Quintero T, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a stakeholder-survey study". Theor Appl Genet 26(2017): 619-629. DOI: 10.2196/48297

Urbanek Z, Zielinski U, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a field trial". Front Plant Sci 28(2020): 630-641. DOI: 10.1007/s00330-025-12031-z

Vasquez A, Alvez V, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a systematic review". Transgenic Res 30(2023): 641-653. DOI: 10.3389/fdgth.2026.1699005

Wojcik H, Hendriksen W, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a confined-trial evaluation". GM Crops Food 32(2014): 652-665. DOI: 10.3389/fdgth.2026.1834800

Zielinski O, Ostrowski Z, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a benchmark comparison". Plant Cell Rep 34(2017): 663-677. DOI: 10.2196/69678

Andric V, Vasquez A, et al. "Biofertiliser Formulations Based on Nitrogen-Fixing Endophytes: a multi-generation study". Mol Plant 36(2020): 674-680. DOI: 10.7759/cureus.46454

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Published

2022-03-20

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Vol. 1 No. 1 (2022): vol 1 issue 1 2022

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