Chloroplast Transformation for Recombinant Protein Yield: A Case Study of Downstream Purification Cost

Authors

  • Domitila Echevarrieta Centre for Molecular Farming, Bajo Aragon Institute of Plant Science, Alcaniz, Spain Author
  • Camiel Jonckheere Department of Bioprocess Engineering, Pays des Collines Centre for Plant Genome Engineering, Frasnes, Belgium Author
  • Franciszek Andrzejewski Department of Plant Biochemistry, Opolszczyzna Centre for Plant Genome Engineering, Opole, Poland Author

DOI:

https://doi.org/10.5281/zenodo.22666368

Keywords:

Chloroplast Transformation for Recombinant Protein Yield; Agricultural biotechnology; Gene editing; Evidence synthesis; Biosafety regulation

Abstract

Chloroplast Transformation for Recombinant Protein Yield has moved quickly from a research idea to something a working
breeding or production programme can actually use, but the evidence behind it has not always kept pace. We pull together the
current evidence for chloroplast transformation for recombinant protein yield, covering the methodological, practical, regulatory
and cost angles, with an eye on where practice has genuinely settled and where researchers and regulators are still largely
working it out for themselves. Selective, context-specific adoption looks better supported by the evidence gathered here than either
broad rollout or blanket avoidance.

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Alvez D, Dumitrescu A, et al. "Chloroplast Transformation for Recombinant Protein Yield: a field trial". Mol Plant 28(2020): 410-419. DOI: 10.7759/cureus.46454

Brankovic K, Kowalczyk B, et al. "Chloroplast Transformation for Recombinant Protein Yield: a systematic review". J Exp Bot 30(2023): 421-431. DOI: 10.1016/j.ssci.2025.107028

Castellani R, Ristic C, et al. "Chloroplast Transformation for Recombinant Protein Yield: a confined-trial evaluation". Crop Sci 32(2014): 432-443. DOI: 10.2196/48633

Dumitrescu A, Andric D, et al. "Chloroplast Transformation for Recombinant Protein Yield: a benchmark comparison". Agron J 34(2017): 443-455. DOI: 10.1371/journal.pone.0305949

Eriksdottir B, Brankovic E, et al. "Chloroplast Transformation for Recombinant Protein Yield: a multi-generation study". Plant Physiol 36(2020): 454-467. DOI: 10.1371/journal.pdig.0000514

Farkas I, Ionescu F, et al. "Chloroplast Transformation for Recombinant Protein Yield: a pilot deployment". New Phytol 38(2023): 465-479. DOI: 10.17219/acem/208596

Grabowski P, Petrescu G, et al. "Chloroplast Transformation for Recombinant Protein Yield: a case study". Nat Biotechnol 40(2014): 476-482. DOI: 10.7759/cureus.104086

Hendriksen W, Wojcik H, et al. "Chloroplast Transformation for Recombinant Protein Yield: a meta-analysis". Sci Rep 42(2017): 487-494. DOI: 10.1038/s41746-023-00852-5

Ionescu F, Foscarini I, et al. "Chloroplast Transformation for Recombinant Protein Yield: a comparative trial". PLoS One 44(2020): 498-506. DOI: 10.1016/j.mayocpiqo.2022.09.004

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Kowalczyk N, Novotna K, et al. "Chloroplast Transformation for Recombinant Protein Yield: a field trial". Biotechnol Adv 8(2014): 520-530. DOI: 10.1093/medlaw/fwad013

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Wojcik H, Hendriksen W, et al. "Chloroplast Transformation for Recombinant Protein Yield: a confined-trial evaluation". GM Crops Food 32(2014): 652-665. DOI: 10.7759/cureus.46454

Zielinski O, Ostrowski Z, et al. "Chloroplast Transformation for Recombinant Protein Yield: a benchmark comparison". Plant Cell Rep 34(2017): 663-677. DOI: 10.1016/j.ssci.2025.107028

Andric V, Vasquez A, et al. "Chloroplast Transformation for Recombinant Protein Yield: a multi-generation study". Mol Plant 36(2020): 674-680. DOI: 10.2196/48633

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Published

2022-08-15

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Vol. 1 No. 2 (2022): vol 1 issue 2 2022

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