Base Editing Without Double-Strand Breaks in Tomato: A Technical Note on Guide Design and Bystander Edits
DOI:
https://doi.org/10.5281/zenodo.22666390Keywords:
Base Editing Without Double-Strand Breaks in Tomato; Agricultural biotechnology; Gene editing; Evidence synthesis; Biosafety regulationAbstract
Base Editing Without Double-Strand Breaks in Tomato has moved quickly from a research idea to something a working breeding
or production programme can actually use, but the evidence behind it has not always kept pace. We pull together the current
evidence for base editing without double-strand breaks in tomato, covering the methodological, practical, regulatory and cost
angles, with an eye on where practice has genuinely settled and where researchers and regulators are still largely working it out
for themselves. Selective, context-specific adoption looks better supported by the evidence gathered here than either broad rollout
or blanket avoidance.
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Castellani R, Ristic C, et al. "Base Editing Without Double-Strand Breaks in Tomato: a confined-trial evaluation". Crop Sci 32(2014): 432-443. DOI: 10.1371/journal.pdig.0000514
Dumitrescu A, Andric D, et al. "Base Editing Without Double-Strand Breaks in Tomato: a benchmark comparison". Agron J 34(2017): 443-455. DOI: 10.17219/acem/208596
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Farkas I, Ionescu F, et al. "Base Editing Without Double-Strand Breaks in Tomato: a pilot deployment". New Phytol 38(2023): 465-479. DOI: 10.1038/s41746-023-00852-5
Grabowski P, Petrescu G, et al. "Base Editing Without Double-Strand Breaks in Tomato: a case study". Nat Biotechnol 40(2014): 476-482. DOI: 10.1016/j.mayocpiqo.2022.09.004
Hendriksen W, Wojcik H, et al. "Base Editing Without Double-Strand Breaks in Tomato: a meta-analysis". Sci Rep 42(2017): 487-494. DOI: 10.1177/08404704241291226
Ionescu F, Foscarini I, et al. "Base Editing Without Double-Strand Breaks in Tomato: a comparative trial". PLoS One 44(2020): 498-506. DOI: 10.1093/medlaw/fwad013
Jankowiak G, Grabowski J, et al. "Base Editing Without Double-Strand Breaks in Tomato: a stakeholder-survey study". BMC Plant Biol 46(2023): 509-518. DOI: 10.2196/89834
Kowalczyk N, Novotna K, et al. "Base Editing Without Double-Strand Breaks in Tomato: a field trial". Biotechnol Adv 8(2014): 520-530. DOI: 10.1007/s10916-024-02104-9
Lindqvist U, Urbanek L, et al. "Base Editing Without Double-Strand Breaks in Tomato: a systematic review". Plant Mol Biol 10(2017): 531-542. DOI: 10.3389/fdgth.2026.1896184
Marchetti D, Doncheva M, et al. "Base Editing Without Double-Strand Breaks in Tomato: a confined-trial evaluation". Field Crops Res 12(2020): 542-554. DOI: 10.3390/diagnostics15162117
Novotna E, Eriksdottir N, et al. "Base Editing Without Double-Strand Breaks in Tomato: a benchmark comparison". Euphytica 14(2023): 553-566. DOI: 10.4103/jrpp.jrpp_20_65
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Urbanek Z, Zielinski U, et al. "Base Editing Without Double-Strand Breaks in Tomato: a field trial". Front Plant Sci 28(2020): 630-641. DOI: 10.7759/cureus.46454
Vasquez A, Alvez V, et al. "Base Editing Without Double-Strand Breaks in Tomato: a systematic review". Transgenic Res 30(2023): 641-653. DOI: 10.1016/j.ssci.2025.107028
Wojcik H, Hendriksen W, et al. "Base Editing Without Double-Strand Breaks in Tomato: a confined-trial evaluation". GM Crops Food 32(2014): 652-665. DOI: 10.2196/48633
Zielinski O, Ostrowski Z, et al. "Base Editing Without Double-Strand Breaks in Tomato: a benchmark comparison". Plant Cell Rep 34(2017): 663-677. DOI: 10.1371/journal.pone.0305949
Andric V, Vasquez A, et al. "Base Editing Without Double-Strand Breaks in Tomato: a multi-generation study". Mol Plant 36(2020): 674-680. DOI: 10.1371/journal.pdig.0000514
