Synthetic Promoters for Tissue-Specific Expression in Crops: A Review of Design and Field Stability

Authors

  • Felizitas Grillmayer Centre for Synthetic Biology, Lavanttal Centre for Plant Genome Engineering, Wolfsberg, Austria Author
  • Edward Calleja Department of Plant Molecular Biology, Zebbug Centre for Plant Genome Engineering, Zebbug, Malta Author
  • Fani Zahariev Centre for Plant Genome Engineering, Strandzha Institute of Agricultural Biotechnology, Malko Tarnovo, Bulgaria Author

DOI:

https://doi.org/10.5281/zenodo.22667090

Keywords:

Synthetic Promoters for Tissue-Specific Expression in Crops; Agricultural biotechnology; Gene editing; Evidence synthesis; Biosafety regulation

Abstract

There is real interest in synthetic promoters for tissue-specific expression in crops right now, and real uncertainty about exactly
where it delivers the most value. We pull together the current evidence for synthetic promoters for tissue-specific expression in
crops, covering the methodological, practical, regulatory and cost angles, with an eye on where practice has genuinely settled and
where researchers and regulators are still largely working it out for themselves. Selective, context-specific adoption looks better
supported by the evidence gathered here than either broad rollout or blanket avoidance.

References

Alvez D, Dumitrescu A, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a field trial". Plant Biotechnol J 8(2014): 80-86. DOI: 10.2196/89834

Brankovic K, Kowalczyk B, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a systematic review". Theor Appl Genet 10(2017): 91-98. DOI: 10.1007/s10916-024-02104-9

Castellani R, Ristic C, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a confined-trial evaluation". Front Plant Sci 12(2020): 102-110. DOI: 10.3389/fdgth.2026.1896184

Dumitrescu A, Andric D, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a benchmark comparison". Transgenic Res 14(2023): 113-122. DOI: 10.3390/diagnostics15162117

Eriksdottir B, Brankovic E, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a multi-generation study". GM Crops Food 16(2014): 124-134. DOI: 10.4103/jrpp.jrpp_20_65

Farkas I, Ionescu F, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a pilot deployment". Plant Cell Rep 18(2017): 135-146. DOI: 10.3389/fdgth.2026.1763884

Grabowski P, Petrescu G, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a case study". Mol Plant 20(2020): 146-158. DOI: 10.2196/48297

Hendriksen W, Wojcik H, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a meta-analysis". J Exp Bot 22(2023): 157-170. DOI: 10.1007/s00330-025-12031-z

Ionescu F, Foscarini I, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a comparative trial". Crop Sci 24(2014): 168-182. DOI: 10.3389/fdgth.2026.1699005

Jankowiak G, Grabowski J, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a stakeholder-survey study". Agron J 26(2017): 179-185. DOI: 10.3389/fdgth.2026.1834800

Kowalczyk N, Novotna K, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a field trial". Plant Physiol 28(2020): 190-197. DOI: 10.2196/69678

Lindqvist U, Urbanek L, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a systematic review". New Phytol 30(2023): 201-209. DOI: 10.7759/cureus.46454

Marchetti D, Doncheva M, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a confined-trial evaluation". Nat Biotechnol 32(2014): 212-221. DOI: 10.1016/j.ssci.2025.107028

Novotna E, Eriksdottir N, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a benchmark comparison". Sci Rep 34(2017): 223-233. DOI: 10.2196/48633

Ostrowski L, Lindqvist O, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a multi-generation study". PLoS One 36(2020): 234-245. DOI: 10.1371/journal.pone.0305949

Petrescu S, Szabo P, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a pilot deployment". BMC Plant Biol 38(2023): 245-257. DOI: 10.1371/journal.pdig.0000514

Quintero B, Balogh Q, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a case study". Biotechnol Adv 40(2014): 256-269. DOI: 10.17219/acem/208596

Ristic C, Castellani R, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a meta-analysis". Plant Mol Biol 42(2017): 267-281. DOI: 10.7759/cureus.104086

Szabo J, Jankowiak S, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a comparative trial". Field Crops Res 44(2020): 278-284. DOI: 10.1038/s41746-023-00852-5

Tarasov Q, Quintero T, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a stakeholder-survey study". Euphytica 46(2023): 289-296. DOI: 10.1016/j.mayocpiqo.2022.09.004

Urbanek Z, Zielinski U, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a field trial". Front Genome Ed 8(2014): 300-308. DOI: 10.1177/08404704241291226

Vasquez A, Alvez V, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a systematic review". CRISPR J 10(2017): 311-320. DOI: 10.1093/medlaw/fwad013

Wojcik H, Hendriksen W, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a confined-trial evaluation". Plant Cell 12(2020): 322-332. DOI: 10.2196/89834

Zielinski O, Ostrowski Z, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a benchmark comparison". Trends Biotechnol 14(2023): 333-344. DOI: 10.1007/s10916-024-02104-9

Andric V, Vasquez A, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a multi-generation study". Plant Biotechnol J 16(2014): 344-356. DOI: 10.3389/fdgth.2026.1896184

Balogh E, Emmerich B, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a pilot deployment". Theor Appl Genet 18(2017): 355-368. DOI: 10.3390/diagnostics15162117

Chiriac F, Farkas C, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a case study". Front Plant Sci 20(2020): 366-380. DOI: 10.4103/jrpp.jrpp_20_65

Doncheva M, Marchetti D, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a meta-analysis". Transgenic Res 22(2023): 377-383. DOI: 10.3389/fdgth.2026.1763884

Emmerich T, Tarasov E, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a comparative trial". GM Crops Food 24(2014): 388-395. DOI: 10.2196/48297

Foscarini C, Chiriac F, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a stakeholder-survey study". Plant Cell Rep 26(2017): 399-407. DOI: 10.1007/s00330-025-12031-z

Alvez D, Dumitrescu A, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a field trial". Mol Plant 28(2020): 410-419. DOI: 10.3389/fdgth.2026.1699005

Brankovic K, Kowalczyk B, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a systematic review". J Exp Bot 30(2023): 421-431. DOI: 10.3389/fdgth.2026.1834800

Castellani R, Ristic C, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a confined-trial evaluation". Crop Sci 32(2014): 432-443. DOI: 10.2196/69678

Dumitrescu A, Andric D, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a benchmark comparison". Agron J 34(2017): 443-455. DOI: 10.7759/cureus.46454

Eriksdottir B, Brankovic E, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a multi-generation study". Plant Physiol 36(2020): 454-467. DOI: 10.1016/j.ssci.2025.107028

Farkas I, Ionescu F, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a pilot deployment". New Phytol 38(2023): 465-479. DOI: 10.2196/48633

Grabowski P, Petrescu G, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a case study". Nat Biotechnol 40(2014): 476-482. DOI: 10.1371/journal.pone.0305949

Hendriksen W, Wojcik H, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a meta-analysis". Sci Rep 42(2017): 487-494. DOI: 10.1371/journal.pdig.0000514

Ionescu F, Foscarini I, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a comparative trial". PLoS One 44(2020): 498-506. DOI: 10.17219/acem/208596

Jankowiak G, Grabowski J, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a stakeholder-survey study". BMC Plant Biol 46(2023): 509-518. DOI: 10.7759/cureus.104086

Kowalczyk N, Novotna K, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a field trial". Biotechnol Adv 8(2014): 520-530. DOI: 10.1038/s41746-023-00852-5

Lindqvist U, Urbanek L, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a systematic review". Plant Mol Biol 10(2017): 531-542. DOI: 10.1016/j.mayocpiqo.2022.09.004

Marchetti D, Doncheva M, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a confined-trial evaluation". Field Crops Res 12(2020): 542-554. DOI: 10.1177/08404704241291226

Novotna E, Eriksdottir N, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a benchmark comparison". Euphytica 14(2023): 553-566. DOI: 10.1093/medlaw/fwad013

Ostrowski L, Lindqvist O, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a multi-generation study". Front Genome Ed 16(2014): 564-578. DOI: 10.2196/89834

Petrescu S, Szabo P, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a pilot deployment". CRISPR J 18(2017): 575-581. DOI: 10.1007/s10916-024-02104-9

Quintero B, Balogh Q, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a case study". Plant Cell 20(2020): 586-593. DOI: 10.3389/fdgth.2026.1896184

Ristic C, Castellani R, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a meta-analysis". Trends Biotechnol 22(2023): 597-605. DOI: 10.3390/diagnostics15162117

Szabo J, Jankowiak S, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a comparative trial". Plant Biotechnol J 24(2014): 608-617. DOI: 10.4103/jrpp.jrpp_20_65

Tarasov Q, Quintero T, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a stakeholder-survey study". Theor Appl Genet 26(2017): 619-629. DOI: 10.3389/fdgth.2026.1763884

Urbanek Z, Zielinski U, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a field trial". Front Plant Sci 28(2020): 630-641. DOI: 10.2196/48297

Vasquez A, Alvez V, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a systematic review". Transgenic Res 30(2023): 641-653. DOI: 10.1007/s00330-025-12031-z

Wojcik H, Hendriksen W, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a confined-trial evaluation". GM Crops Food 32(2014): 652-665. DOI: 10.3389/fdgth.2026.1699005

Zielinski O, Ostrowski Z, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a benchmark comparison". Plant Cell Rep 34(2017): 663-677. DOI: 10.3389/fdgth.2026.1834800

Andric V, Vasquez A, et al. "Synthetic Promoters for Tissue-Specific Expression in Crops: a multi-generation study". Mol Plant 36(2020): 674-680. DOI: 10.2196/69678

Downloads

Published

2025-03-25