Apomixis Engineering for Hybrid Seed Systems: A Review of Synthetic Routes and Remaining Barriers

Authors

  • Gilbert Hemmen Centre for Synthetic Biology, Attert Valley Institute of Agricultural Biotechnology, Redange, Luxembourg Author
  • Fredrikke Jorstad Department of Plant Breeding, Sunnhordland Centre for Plant Genome Engineering, Stord, Norway Author
  • Inacio Homem Department of Seed Technology, Charneca Centre for Plant Genome Engineering, Coruche, Portugal Author

DOI:

https://doi.org/10.5281/zenodo.22666711

Keywords:

Apomixis Engineering for Hybrid Seed Systems; Agricultural biotechnology; Gene editing; Evidence synthesis; Biosafety regulation

Abstract

There is real interest in apomixis engineering for hybrid seed systems right now, and real uncertainty about exactly where it
delivers the most value. We pull together the current evidence for apomixis engineering for hybrid seed systems, covering the
methodological, practical, regulatory and cost angles, with an eye on where practice has genuinely settled and where researchers
and regulators are still largely working it out for themselves. Selective, context-specific adoption looks better supported by the
evidence gathered here than either broad rollout or blanket avoidance.

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Alvez D, Dumitrescu A, et al. "Apomixis Engineering for Hybrid Seed Systems: a field trial". Mol Plant 28(2020): 410-419. DOI: 10.3389/fdgth.2026.1834800

Brankovic K, Kowalczyk B, et al. "Apomixis Engineering for Hybrid Seed Systems: a systematic review". J Exp Bot 30(2023): 421-431. DOI: 10.2196/69678

Castellani R, Ristic C, et al. "Apomixis Engineering for Hybrid Seed Systems: a confined-trial evaluation". Crop Sci 32(2014): 432-443. DOI: 10.7759/cureus.46454

Dumitrescu A, Andric D, et al. "Apomixis Engineering for Hybrid Seed Systems: a benchmark comparison". Agron J 34(2017): 443-455. DOI: 10.1016/j.ssci.2025.107028

Eriksdottir B, Brankovic E, et al. "Apomixis Engineering for Hybrid Seed Systems: a multi-generation study". Plant Physiol 36(2020): 454-467. DOI: 10.2196/48633

Farkas I, Ionescu F, et al. "Apomixis Engineering for Hybrid Seed Systems: a pilot deployment". New Phytol 38(2023): 465-479. DOI: 10.1371/journal.pone.0305949

Grabowski P, Petrescu G, et al. "Apomixis Engineering for Hybrid Seed Systems: a case study". Nat Biotechnol 40(2014): 476-482. DOI: 10.1371/journal.pdig.0000514

Hendriksen W, Wojcik H, et al. "Apomixis Engineering for Hybrid Seed Systems: a meta-analysis". Sci Rep 42(2017): 487-494. DOI: 10.17219/acem/208596

Ionescu F, Foscarini I, et al. "Apomixis Engineering for Hybrid Seed Systems: a comparative trial". PLoS One 44(2020): 498-506. DOI: 10.7759/cureus.104086

Jankowiak G, Grabowski J, et al. "Apomixis Engineering for Hybrid Seed Systems: a stakeholder-survey study". BMC Plant Biol 46(2023): 509-518. DOI: 10.1038/s41746-023-00852-5

Kowalczyk N, Novotna K, et al. "Apomixis Engineering for Hybrid Seed Systems: a field trial". Biotechnol Adv 8(2014): 520-530. DOI: 10.1016/j.mayocpiqo.2022.09.004

Lindqvist U, Urbanek L, et al. "Apomixis Engineering for Hybrid Seed Systems: a systematic review". Plant Mol Biol 10(2017): 531-542. DOI: 10.1177/08404704241291226

Marchetti D, Doncheva M, et al. "Apomixis Engineering for Hybrid Seed Systems: a confined-trial evaluation". Field Crops Res 12(2020): 542-554. DOI: 10.1093/medlaw/fwad013

Novotna E, Eriksdottir N, et al. "Apomixis Engineering for Hybrid Seed Systems: a benchmark comparison". Euphytica 14(2023): 553-566. DOI: 10.2196/89834

Ostrowski L, Lindqvist O, et al. "Apomixis Engineering for Hybrid Seed Systems: a multi-generation study". Front Genome Ed 16(2014): 564-578. DOI: 10.1007/s10916-024-02104-9

Petrescu S, Szabo P, et al. "Apomixis Engineering for Hybrid Seed Systems: a pilot deployment". CRISPR J 18(2017): 575-581. DOI: 10.3389/fdgth.2026.1896184

Quintero B, Balogh Q, et al. "Apomixis Engineering for Hybrid Seed Systems: a case study". Plant Cell 20(2020): 586-593. DOI: 10.3390/diagnostics15162117

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Andric V, Vasquez A, et al. "Apomixis Engineering for Hybrid Seed Systems: a multi-generation study". Mol Plant 36(2020): 674-680. DOI: 10.7759/cureus.46454

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Published

2025-07-10