Insect Cell Culture for Veterinary Vaccine Antigens: A Review of Platform Choice and Yield

Authors

  • Delfina Beccalossi Centre for Veterinary Vaccines, Chianti Centre for Plant Genome Engineering, Greve, Italy Author
  • Anatole Montagnac Centre for Insect Biotechnology, Medoc Institute of Plant Science, Pauillac, France Author
  • Iztok Erjavec Department of Bioprocess Engineering, Vipava Institute of Agricultural Biotechnology, Ajdovscina, Slovenia Author

DOI:

https://doi.org/10.5281/zenodo.22666764

Keywords:

Insect Cell Culture for Veterinary Vaccine Antigens; Agricultural biotechnology; Gene editing; Evidence synthesis; Biosafety regulation

Abstract

There is real interest in insect cell culture for veterinary vaccine antigens right now, and real uncertainty about exactly where it
delivers the most value. We pull together the current evidence for insect cell culture for veterinary vaccine antigens, covering the
methodological, practical, regulatory and cost angles, with an eye on where practice has genuinely settled and where researchers
and regulators are still largely working it out for themselves. Selective, context-specific adoption looks better supported by the
evidence gathered here than either broad rollout or blanket avoidance.

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Foscarini C, Chiriac F, et al. "Insect Cell Culture for Veterinary Vaccine Antigens: a stakeholder-survey study". Plant Cell Rep 26(2017): 399-407. DOI: 10.2196/48633

Alvez D, Dumitrescu A, et al. "Insect Cell Culture for Veterinary Vaccine Antigens: a field trial". Mol Plant 28(2020): 410-419. DOI: 10.1371/journal.pone.0305949

Brankovic K, Kowalczyk B, et al. "Insect Cell Culture for Veterinary Vaccine Antigens: a systematic review". J Exp Bot 30(2023): 421-431. DOI: 10.1371/journal.pdig.0000514

Castellani R, Ristic C, et al. "Insect Cell Culture for Veterinary Vaccine Antigens: a confined-trial evaluation". Crop Sci 32(2014): 432-443. DOI: 10.17219/acem/208596

Dumitrescu A, Andric D, et al. "Insect Cell Culture for Veterinary Vaccine Antigens: a benchmark comparison". Agron J 34(2017): 443-455. DOI: 10.7759/cureus.104086

Eriksdottir B, Brankovic E, et al. "Insect Cell Culture for Veterinary Vaccine Antigens: a multi-generation study". Plant Physiol 36(2020): 454-467. DOI: 10.1038/s41746-023-00852-5

Farkas I, Ionescu F, et al. "Insect Cell Culture for Veterinary Vaccine Antigens: a pilot deployment". New Phytol 38(2023): 465-479. DOI: 10.1016/j.mayocpiqo.2022.09.004

Grabowski P, Petrescu G, et al. "Insect Cell Culture for Veterinary Vaccine Antigens: a case study". Nat Biotechnol 40(2014): 476-482. DOI: 10.1177/08404704241291226

Hendriksen W, Wojcik H, et al. "Insect Cell Culture for Veterinary Vaccine Antigens: a meta-analysis". Sci Rep 42(2017): 487-494. DOI: 10.1093/medlaw/fwad013

Ionescu F, Foscarini I, et al. "Insect Cell Culture for Veterinary Vaccine Antigens: a comparative trial". PLoS One 44(2020): 498-506. DOI: 10.2196/89834

Jankowiak G, Grabowski J, et al. "Insect Cell Culture for Veterinary Vaccine Antigens: a stakeholder-survey study". BMC Plant Biol 46(2023): 509-518. DOI: 10.1007/s10916-024-02104-9

Kowalczyk N, Novotna K, et al. "Insect Cell Culture for Veterinary Vaccine Antigens: a field trial". Biotechnol Adv 8(2014): 520-530. DOI: 10.3389/fdgth.2026.1896184

Lindqvist U, Urbanek L, et al. "Insect Cell Culture for Veterinary Vaccine Antigens: a systematic review". Plant Mol Biol 10(2017): 531-542. DOI: 10.3390/diagnostics15162117

Marchetti D, Doncheva M, et al. "Insect Cell Culture for Veterinary Vaccine Antigens: a confined-trial evaluation". Field Crops Res 12(2020): 542-554. DOI: 10.4103/jrpp.jrpp_20_65

Novotna E, Eriksdottir N, et al. "Insect Cell Culture for Veterinary Vaccine Antigens: a benchmark comparison". Euphytica 14(2023): 553-566. DOI: 10.3389/fdgth.2026.1763884

Ostrowski L, Lindqvist O, et al. "Insect Cell Culture for Veterinary Vaccine Antigens: a multi-generation study". Front Genome Ed 16(2014): 564-578. DOI: 10.2196/48297

Petrescu S, Szabo P, et al. "Insect Cell Culture for Veterinary Vaccine Antigens: a pilot deployment". CRISPR J 18(2017): 575-581. DOI: 10.1007/s00330-025-12031-z

Quintero B, Balogh Q, et al. "Insect Cell Culture for Veterinary Vaccine Antigens: a case study". Plant Cell 20(2020): 586-593. DOI: 10.3389/fdgth.2026.1699005

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Vasquez A, Alvez V, et al. "Insect Cell Culture for Veterinary Vaccine Antigens: a systematic review". Transgenic Res 30(2023): 641-653. DOI: 10.2196/48633

Wojcik H, Hendriksen W, et al. "Insect Cell Culture for Veterinary Vaccine Antigens: a confined-trial evaluation". GM Crops Food 32(2014): 652-665. DOI: 10.1371/journal.pone.0305949

Zielinski O, Ostrowski Z, et al. "Insect Cell Culture for Veterinary Vaccine Antigens: a benchmark comparison". Plant Cell Rep 34(2017): 663-677. DOI: 10.1371/journal.pdig.0000514

Andric V, Vasquez A, et al. "Insect Cell Culture for Veterinary Vaccine Antigens: a multi-generation study". Mol Plant 36(2020): 674-680. DOI: 10.17219/acem/208596

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Published

2026-02-20